A benzimidazole-based ratiometric fluorescent probe for the accurate and rapid monitoring of lysosomal pH in cell autophagy and anticounterfeiting

Abstract

The change of lysosomal pH is an important physiological indicator in the process of cell autophagy. Herein, a ratiometric fluorescent probe 4-(2-(1H-benzo[d]imidazol-2-yl)vinyl)-N,N-dimethylaniline (BD) has been synthesized and applied for lysosomal...

Ratiometric fluorescent probes for pH mapping in cellular organelles

“…8) for the detection of lysosomal ΔpHi during autophagy. 145 Here, both the imidazole and dimethylamino groups could target lysosomes, and the benzimidazole group acted as a proton receptor for ratiometric fluorescence emission with a p K a of 4.73. Lin et al designed three new intramolecular charge transfer ratiometric fluorophores by modifying different alkyl chains on a dye.…”

In vivo measurement of autophagic flux by fluorescence correlation spectroscopy

“…S12 †). 21,32 Then, the EGFP signal would be quenched in the lysosomal acidic environment, and autophagosomes as well as the components in the autophagosome (including EGFP and other proteins) are degraded. 7 Here, in the presence of 10 μM CQ, we assessed autophagic flux by the P AP value, which reflects the number of autophagosomes.…”