The identification of people's gender and events in our everyday applications by means of gait knowledge is becoming important. Security, safety, entertainment, and billing are the examples of such applications. Many technologies could also be used to monitor people's gender and activities. Existing solutions and applications are subject to the privacy and the implementation costs and the accuracy they have achieved. For instance, CCTV or Kinect sensor technology for people is a violation of privacy, since most people don't want to make their photos or videos during their daily work. A new addition to the gait analysis field is the inertial sensor-based gait dataset. Therefore, in this paper, we have classified people's gender from an inertial sensor-based gait dataset. We have collected the gait dataset from Osaka University. Four machine learning algorithms have been applied to identify people's gender and they are Support Vector Machine (SVM), K-Nearest Neighbor (KNN), Bagging, and Boosting algorithm. For classifying gender, some useful features are extracted from the raw data, and 84 features have been used to identify people's gender. After feature selection the experimental outcome exhibits the accuracy of gender identification via the Bagging stands at around 87.858%, while it is about 86.09% via SVM. This will in turn form the basis to support human wellbeing by using gait knowledge.