A B-1a cell subset induces Foxp3− T cells with regulatory activity through an IL-10-independent pathway

Hsu, Ling-Hui; Li, Kun-Po; Chu, Kuan-Hua; Chiang, Bor‐Luen

doi:10.1038/cmi.2014.56

Cited by 46 publications

(59 citation statements)

References 40 publications

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“…Previous in vitro cell culture system demonstrated that splenic B cells required cell-cell contact including CD80 and CD86 costimulation for Treg-of-B cells induction29. In consistent with these, B-cell-induced T cell generated with additional anti-CD28 antibody decreased suppressive ability53.…”

Section: Discussionmentioning

confidence: 82%

Characterization of c-Maf+Foxp3− Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

Chien

Chen

et al. 2017

Sci Rep

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The role of B cells in the development of CD4+ regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4+CD25− T cells into CD4+CD25+Foxp3− T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4+Foxp3− regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function.

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Section: Discussionmentioning

confidence: 82%

Characterization of c-Maf+Foxp3− Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

Chien

Chen

et al. 2017

Sci Rep

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“…37 In the following passages, we cite three methods for the induction of Tr1 cells to illustrate critical factors involved in the process of Tr1-cell differentiation and to provide useful information for the study of Tr1 cells.…”

Section: In Vitro Induction Of Tr1 Cellsmentioning

confidence: 99%

Type 1 regulatory T cells: a new mechanism of peripheral immune tolerance

et al. 2015

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“…Breg cells exhibit immunosuppressive function via diverse regulatory mechanisms. In addition to schistosome-induced splenic B cells, B-1a cells from the peritoneal cavity (PerC) also have the regulatory capability to induce Treg cells [20]. Moreover, it has been suggested that B cells are essential for Th2 response during infection with S. mansoni [21].…”

Section: Introductionmentioning

confidence: 99%

B cells induced by Schistosoma japonicum infection display diverse regulatory phenotypes and modulate CD4+ T cell response

et al. 2020

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Background: The increased activity of regulatory B cells (Breg) is known to be involved in immunosuppression during helminth infection, which is characterized by inducing IL-10-producing Breg cells. However, the current knowledge of B cell subsets differentiation and IL-10-independent immunoregulatory mechanisms of B cells in schistosomiasis is insufficient. Methods: BALB/c mice were percutaneously infected with cercariae for investigating the profile of B cell subsets during Schistosoma japonicum infection. B cells isolated from the spleen or peritoneal cavity were analyzed for the regulatory phenotype after stimulation with soluble egg antigens (SEA) in vitro. CD4 + T cells were then cocultured with B cells pretreated with or without anti-PD-L1 antibody for investigating the role of B cells from infected mice on regulating CD4 + T cells. Furthermore, the in vivo administration of anti-PD-L1 antibody was conducted to investigate the role of PD-L1 in regulating host immunity during infection. Results: The percentages of peritoneal and splenic B-1a cells, as well as marginal zone B (MZB) cells were decreased at eight and twelve weeks after infection compared to those from uninfected mice. In splenic B cells, TGF-β expression was increased at eight weeks but declined at twelve weeks of infection, and PD-L1 expression was elevated at both eight and twelve weeks of infection. In addition, SEA stimulation in vitro significantly promoted the expression of IL-10 in peritoneal B cells and CD5 in splenic B cells, and the SEA-stimulated splenic and peritoneal B cells preferentially expressed PD-L1 and TGF-β. The splenic B cells from infected mice were able to suppress the function of Th1 and Th2 cells in vitro but to expand the expression of Tfh transcription factor Bcl6, which was further enhanced by blocking PD-L1 of B cells before co-cultivation. Moreover, Th2 response and Bcl6 expression in CD4 + T cells were also increased in vivo by blocking PD-L1 after infection, although the hepatic pathology was slightly influenced. Conclusions: Our findings revealed that S. japonicum infection modulates the differentiation of B cell subsets that have the capability to affect the CD4 + T cell response. This study contributes to a better understanding of B cells immune response during schistosomiasis.

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A B-1a cell subset induces Foxp3− T cells with regulatory activity through an IL-10-independent pathway

Cited by 46 publications

References 40 publications

Characterization of c-Maf+Foxp3− Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

Characterization of c-Maf+Foxp3− Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

Type 1 regulatory T cells: a new mechanism of peripheral immune tolerance

B cells induced by Schistosoma japonicum infection display diverse regulatory phenotypes and modulate CD4+ T cell response

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