A 5′P degradation hot spot influences molecular farming of anticancerogenic nuclease TBN1 in tobacco cells

Týcová, Anna; Piernikarczyk, Rajen J. J.; Kugler, Michaël; Lipovová, Petra; Podzimek, T.; Steger, Gerhard; Matoušek, Jaroslav

doi:10.1007/s11240-016-1054-x

Cited by 2 publications

(1 citation statement)

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“…An air-lift type bioreactor (130 L) is another efficient system used to produce recombinant proteins such as the B subunit of E. coli heat-labile toxin, which accumulated to 0.36% TSP in Siberian ginseng somatic embryos [103]. When expression of tomato bifunctional nuclease1 was compared between N. benthamiana and BY-2 cell suspension cultures, the suspension culture showed stable maintenance with lower expression than in N. benthamiana [104]. The most impressive achievement to date from a cell culture system using a tank bioreactor is the first plant-produced commercial therapeutic protein, produced in carrot cells grown in a flexible polyethylene disposable bioreactor (www.protalix.com).…”

Section: Bioreactor Culture Systemsmentioning

confidence: 99%

Development of Systems for the Production of Plant-Derived Biopharmaceuticals

Moon

Park

et al. 2019

Plants

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Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.

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Section: Bioreactor Culture Systemsmentioning

confidence: 99%