A 3D-printed robotic system for fully automated multiparameter analysis of drinkable water samples

Almeida, Pedro Lemos de; Lima, Lidiane Mâcedo Alves de; Almeida, Luciano F.

doi:10.1016/j.aca.2021.338491

Cited by 5 publications

(2 citation statements)

References 49 publications

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“… 31 For instance, automatic water analysis was achieved with a 3D-printed robotic system coupled with a syringe pump to transfer samples and reagents, temperature and conductivity sensors to provide accurate measurement, and a webcam for colorimetric pH analysis on a 96-well plate. 32 Moreover, a robotic system was used to perform automated ELISA protocols for chloramphenicol detection using colorimetric measurements. 33 Protocols were conducted on a microfluidic chip, where pneumatically driven loading, mixing, and washing steps were achieved with a robotic arm.…”

Section: Introductionmentioning

confidence: 99%

“…LOC devices can combine fluidics, electronics, and optics on a single device for standalone operation. , Automated fluidic operations on these devices require valves, mixers, and pumps that can create difficulties in integrating and controlling all these elements realized with a complex fabrication process. , Electromechanical LOC devices can provide self-driving operations with electronic and mechanical components for fully automated assay protocols . For instance, automatic water analysis was achieved with a 3D-printed robotic system coupled with a syringe pump to transfer samples and reagents, temperature and conductivity sensors to provide accurate measurement, and a webcam for colorimetric pH analysis on a 96-well plate . Moreover, a robotic system was used to perform automated ELISA protocols for chloramphenicol detection using colorimetric measurements .…”

Section: Introductionmentioning

confidence: 99%

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An Electromechanical Lab-on-a-Chip Platform for Colorimetric Detection of Serum Creatinine

et al. 2022

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Chronic kidney disease (CKD) is a high-cost disease that affects approximately one in ten people globally, progresses rapidly, results in kidney failure or dialysis, and triggers other diseases. Although clinically used serum creatinine tests are used to evaluate kidney functions, these tests are not suitable for frequent and regular control at-home settings that obstruct the regular monitoring of kidney functions, improving CKD management with early intervention. This study introduced a new electromechanical lab-on-a-chip platform for point-of-care detection of serum creatinine levels using colorimetric enzyme-linked immunosorbent assay (ELISA). The platform was composed of a chip containing microreservoirs, a stirring bar coated with creatinine-specific antibodies, and a phone to detect color generated via ELISA protocols to evaluate creatinine levels. An electromechanical system was used to move the stirring bar to different microreservoirs and stir it inside them to capture and detect serum creatinine in the sample. The presented platform allowed automated analysis of creatinine in ∼50 min down to ∼1 and ∼2 mg/dL in phosphate-buffered saline (PBS) and fetal bovine serum (FBS), respectively. Phone camera measurements in hue, saturation, value (HSV) space showed sensitive analysis compared to a benchtop spectrophotometer that could allow low-cost analysis at point-of-care.

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Section: Introductionmentioning

confidence: 99%