A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus

Bahçecioğlu, Gökhan; Hasırcı, Nesrin; Bilgen, Bahar; Hasırcı, Vasıf

doi:10.1088/1758-5090/aaf707

Cited by 99 publications

(113 citation statements)

References 53 publications

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“…The highest cell viability was on PCL, although addition of hydrogels had no significant effect on cell viability. Our results are in line with the previous studies reporting around 80% cell viability on the PCL-Ag and PCL-GelMA constructs at Day 1 [23], Day 7 [37] or Day 56 [26] of incubation after printing. Immunostaining of the whole PCL-hydrogel constructs revealed low collagen deposition on PCL and PCL-Ag (Fig.…”

Section: Effects Of Hydrogel Incorporation On Cell Viability and Ecmsupporting

confidence: 93%

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Bahçecioğlu

Bilgen

Hasırcı

et al. 2019

Preprint

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A PCL/hydrogel construct that would mimic the structural organization, biochemistry and anatomy of meniscus was engineered. The compressive (380 ± 40 kPa) and tensile modulus (18.2 ± 0.9 MPa) of the PCL scaffolds were increased significantly when constructs were printed with a shifted design and circumferential strands mimicking the collagen organization in native tissue (p<0.05). Gene expression of human fibrochondrocytes in agarose (Ag), gelatin methacrylate (GelMA), and GelMA-Ag hydrogels was significantly higher than that of cells on the PCL scaffolds after a 21-day culture. GelMA exhibited the highest level of collagen type I (COL1A2) mRNA expression, while GelMA-Ag exhibited the highest level of aggrecan (AGG) expression (p<0.001, compared to PCL). GelMA and GelMA-Ag exhibited a high level of collagen type II (COL2A1) expression (p<0.05, compared to PCL). Anatomical scaffolds with circumferential PCL strands were impregnated with cell-loaded GelMA in the periphery and GelMA-Ag in the inner region. GelMA and GelMA-Ag hydrogels enhanced the production of COL 1 and COL 2 proteins after a 6-week culture (p<0.05). COL 1 expression increased gradually towards the outer periphery, while COL 2 expression decreased. We were thus able to engineer an anatomical meniscus with a cartilage-like inner region and fibrocartilage-like outer region.

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Section: Effects Of Hydrogel Incorporation On Cell Viability and Ecmsupporting

confidence: 93%

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Bahçecioğlu

Bilgen

Hasırcı

et al. 2019

Preprint

Self Cite

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“…Fuller et al found that the inner meniscus was more cartilaginous containing more GAG and expressing more aggrecan and type II collagen than the outer zones . Furthermore, some researches were able to engineer a meniscus, which is cartilage‐like cells with primarily type II collagen at the inner portion and fibrocartilage‐like cells with primarily type I collagen at the outer portion for total meniscus replacement . MeSCs are the only stem cells that have been found in meniscus tissue .…”

Section: Discussionmentioning

confidence: 99%

Characterization and Comparison of Postnatal Rat Meniscus Stem Cells at Different Developmental Stages

Ruan

Chen

et al. 2019

Stem Cells Translational Medicine

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Meniscus-derived stem cells (MeSCs) are a potential cell source for meniscus tissue engineering. The stark morphological and structural changes of meniscus tissue during development indicate the complexity of MeSCs at different tissue regions and stages of development. In this study, we characterized and compared postnatal rat meniscus tissue and MeSCs at different tissue regions and stages of development. We observed that the rat meniscus tissue exhibited marked changes in tissue morphology during development, with day 7 being the most representative time point of different developmental stages. All rat MeSCs displayed typical stem cell characteristics. Rat MeSCs derived from day 7 inner meniscus tissue exhibited the highest self-renewal capacity, cell proliferation, differentiation potential toward various mesenchymal lineage and the highest expression levels of chondrogenic genes and proteins. Transplantation of rat MeSCs derived from day 7 inner meniscus tissue promoted neo-tissue formation and effectively protected joint surface cartilage in vivo. Our results demonstrated for the first time that rat MeSCs are not necessarily better at earlier developmental stages, and that rat MeSCs derived from day 7 inner meniscus tissue may be a superior cell source for effective meniscus regeneration and articular cartilage protection. This information could make a significant contribution to human meniscus tissue engineering in the future. STEM CELLS

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“…[235] Poly(ε-caprolactone) shows versatility in the design of other human tissues undergoing mechanical stress; it has been employed as core material for the realization of an artificial meniscus. [241] The realization of an artificial meniscus is challenging, due to its bizonal structure; the tissue presents a fibrous outer portion, with a cartilaginous counterpart in the inner Figure 10B) allowing for a prototype of a bionic eye. [242] The effects of temperature and viscosity of the 3D printed ink are also of remarkable importance.…”

Section: Biocompatible Scaffoldsmentioning

confidence: 99%

“…Phosphate buffer solution Gelatin solution / Stereolithography [209] - Tissue engineering (meniscus) Organic neat Poly(ε-caprolactone) / Stereolithography [241]…”

Section: Aqueousmentioning

confidence: 99%

Artificial Biosystems by Printing Biology

Arrabito

Ferrara

Bonasera

et al. 2020

Small

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The continuous progress of printing technologies over the past 20 years has fueled the development of a wide plethora of applications in materials sciences, flexible electronics and biotechnologies. More recently, printing methodologies have started up to explore the world of Artificial Biology, offering new paradigms in the direct assembly of Artificial Biosystems (small condensates, compartments, networks, tissues and organs) by mimicking the result of the evolution of living systems and also by redesigning natural biological systems, taking inspiration from them. This recent progress is reported in terms of a new field here defined as Printing Biology, resulting from the intersection between the field of printing and the bottom up Synthetic Biology. Printing Biology explores new approaches for the reconfigurable assembly of designed lifelike or life-inspired structures. This review presents this emerging field, highlighting its main features, i.e. printing methodologies (from 2D to 3D), molecular ink properties, deposition mechanisms, and finally the applications and future challenges. Printing Biology is expected to show a growing impact on the development of biotechnology and lifeinspired fabrication. Printing Biology employs different technologies dispensing molecular inks with tunable composition (molecules, polymers, biomolecules) and drop sizes (from nano-up to macroscale) onto solids or into liquids to develop lifelike or life-inspired artificial biosystems (from small condensates, to compartments up to networks, tissues and organs). This work reviews the extraordinary potential of this emerging research field.

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A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus

Cited by 99 publications

References 53 publications

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Characterization and Comparison of Postnatal Rat Meniscus Stem Cells at Different Developmental Stages

Artificial Biosystems by Printing Biology

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