Characterization and Comparison of Postnatal Rat Meniscus Stem Cells at Different Developmental Stages

Su, He; Ruan, Dengfeng; Chen, Yangwu; Ran, Jisheng; Chen, Xiao; Yin, Zi; Tang, Chaoying; Huang, Jichao; Heng, Boon Chin; Chen, Jialin; Chen, Weishan; Shen, Wei

doi:10.1002/sctm.19-0125

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…One previous study characterized and compared meniscal cell changes in postnatal rat menisci at different developmental stages and showed that the number of cells decreased with maturity, with the highest cell density observed during the early postnatal phase. Moreover, meniscal cells observed during the early postnatal phase showed stem cell characteristics with high self-renewal capacity, cell proliferation, and differentiation potential ( He et al, 2019 ). Thirdly, menisci are known to undergo structural transformation and alterations in the ECM composition with maturity.…”

Section: Discussionmentioning

confidence: 99%

Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears

Yan

Dai

Cheng

et al. 2021

Front. Cell Dev. Biol.

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Healing outcomes of meniscal repair are better in younger than in older. However, exact mechanisms underlying superior healing potential in younger remain unclear from a histological perspective. This study included 24 immature rabbits and 24 mature rabbits. Tears were created in the anterior horn of medial meniscus of right knee in each rabbit. Animals were sacrificed at 1, 3, 6, and 12 weeks postoperatively. We performed macroscopic and histological evaluations of post-meniscal repair specimens. Cells were counted within a region of interest to confirm cellularization at tear site in immature menisci. The width of cell death zone was measured to determine the region of cell death in mature menisci. Apoptosis was evaluated by TUNEL assay. Vascularization was assessed by CD31 immunofluorescence. The glycosaminoglycans and the types 1 and 2 collagen content was evaluated by calculating average optical density of corresponding histological specimens. Cartilage degeneration was also evaluated. Healing outcomes following untreated meniscal tears were superior in immature group. Recellularization with meniscus-like cell morphology was observed at tear edge in immature menisci. Superior recellularization was observed at meniscal sites close to joint capsule than at sites distant from the capsule. Recellularization did not occur at tear site in mature group; however, we observed gradual enlargement of cell death zone. Apoptosis was presented at 1, 3, 6, 12 weeks in immature and mature menisci after untreated meniscal tears. Vascularization was investigated along the tear edges in immature menisci. Glycosaminoglycans and type 2 collagen deposition were negatively affected in immature menisci. We observed glycosaminoglycan degradation in mature menisci and cartilage degeneration, specifically in immature cartilage of the femoral condyle. In conclusion, compared with mature rabbits, immature rabbits showed more robust healing response after untreated meniscal tears. Vascularization contributed to the recellularization after meniscal tears in immature menisci. Meniscal injury fundamentally alters extracellular matrix deposition.

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Section: Discussionmentioning

confidence: 99%

Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears

Yan

Dai

Cheng

et al. 2021

Front. Cell Dev. Biol.

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“…Diaminobenzidine, a chromogen, was used to develop the color for CD11b+, MMP‐13+, and IL‐1β+ staining. To determine the presence of progenitor cells near the injury site, the sections were immunohistochemically stained with CD44 (sc‐7946, Santa Cruz Biotechnology) and CD73 (sc‐25603, Santa Cruz Biotechnology), since progenitor cells in the rat meniscus have been shown to possess CD44 and CD73 marker 29 . Ki‐67 (ab16667, Abcam) was used to assess the proliferation of progenitor cells.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the presence of progenitor cells near the injury site, the sections were immunohistochemically stained with CD44 (sc-7946, Santa Cruz Biotechnology) and CD73 (sc-25603, Santa Cruz Biotechnology), since progenitor cells in the rat meniscus have been shown to possess CD44 and CD73 marker. 29 Ki-67 (ab16667, Abcam) was used to assess the proliferation of progenitor cells. PDGF responsive cell type was determined by the expression of PDGF α-receptors (PDGFRα) and β-receptors (PDGFRβ) on the progenitor cells (ab32570, Abcam).…”

Section: Histology and Immunohistochemistry (Ihc)mentioning

confidence: 99%

Biomolecule‐releasing bioadhesive for glenoid labrum repair through induced host progenitor cell responses

Nguyên

Vaish

et al. 2022

Journal Orthopaedic Research

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Glenoid labral tears occur with repetitive dislocation events and are common injuries observed in shoulder arthroscopic procedures. Although surgery can restore shoulder anatomy, repair is associated with poor clinical outcomes, which may be attributed to the poor regenerative capability of glenoid labral fibrocartilage. Thus, this study was designed to assess whether in situ tissue regeneration via biomolecule‐stimulated recruitment of progenitor cells is a viable approach for the regeneration of labral tears. We developed a click chemistry‐based bioadhesive to improve labral repair and reduce local inflammatory responses due to trauma. Additionally, we previously identified the presence of progenitor cells in the human labrum, which can be recruited by platelet‐derived growth factor (PDGF). Thus, we hypothesized that PDGF‐releasing adhesives could induce the regenerative responses of progenitor cells at the injury site to improve labral healing. In a rat glenoid labral tear model, we evaluated the effect of PDGF‐releasing adhesives on promoting progenitor cells to participate in labral tear healing. After 3 and 6 weeks, the labrum was histologically analyzed for inflammatory responses, progenitor cell recruitment, proliferation, and extracellular matrix (ECM) production (collagen and glycosaminoglycan). Our results showed that adhesives alone considerably reduced local inflammatory responses and labral tissue dissolution. PDGF‐releasing adhesives significantly increased progenitor cell recruitment, proliferation, and ECM production. These results demonstrate that by accelerating autologous progenitor cell responses, PDGF‐releasing adhesives represent a novel clinically relevant strategy to improve the healing of glenoid labral tears.

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“…The critical liquid-carrying flow rate of gas can be calculated using the liquid film model as follows: based on the minimum shear stress theory and the critical flow rate model of annular mist flow, it is assumed that there is no liquid droplet entrainment and the liquid film is uniformly distributed around the gas core. The dimensionless expression for the shear stress at the annular flow interface is given by (Shen et al, 2019b):…”

Section: Critical Liquid-carrying Flow Rate Modelmentioning

confidence: 99%

Prediction of dynamic liquid level in water-producing shale gas wells based on liquid film model

Nie,

Qiao,

Wang

et al. 2023

Front. Earth Sci.

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The accumulation of liquid in shale gas wells will lead to an increase in bottom-hole pressure, and a decrease in production, even the shutdown of gas wells. Accurately predicting the variation of liquid height in shale gas wellbore and the corresponding production is helpful to determine the liquid accumulation status and the development of reasonable drainage construction plans. It can provide theoretical support for the efficient and stable production of shale gas wells. A predictive analysis model of shale gas wellbore liquid accumulation was established based on a liquid film carrying model, which considers the coupling of flow in the reservoir and wellbore in shale gas production system. This model can accurately predict the liquid accumulation height, production rate, and the bottomhole pressure changes in liquid-accumulating gas wells. A comparative analysis was conducted on the effect of wellbore structure on liquid accumulation characteristics in the typical upward-type and downward-type horizontal wells in the W block. In upward-type horizontal wells, the liquid height increases slowly before the liquid accumulation fills the horizontal section, and the liquid height increases rapidly after the liquid accumulation fills the horizontal section. Compared to upward-type horizontal wells, the liquid height rises more quickly and the gas production rate is more sensitive to liquid accumulation in downward-type horizontal wells.

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Characterization and Comparison of Postnatal Rat Meniscus Stem Cells at Different Developmental Stages

Cited by 9 publications

References 57 publications

Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears

Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears

Biomolecule‐releasing bioadhesive for glenoid labrum repair through induced host progenitor cell responses

Prediction of dynamic liquid level in water-producing shale gas wells based on liquid film model

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