A 3D Biocompatible Plasmonic Tweezer for Single Cell Manipulation

Abstract

The data that support the findings of this study are available in the Supporting Information of this article.

Design and full analysis of a metafiber-based photothermal bio-probe with perfect conversion efficiency

“…By varying S from 320 nm to 510 nm, we simulated the absorption of the metafiber absorber with different W and L at a wavelength of 1310 nm, as shown in figure 3(a). With increasing S, the width of the absorption peak gradually becomes wider, and the peak position moves in the direction of decreasing L or increasing W. When S = 470 nm, the structure can achieve the maximum absorption at specific W and L, which correspond to the result that estimated by equation (9). The relationship between W and L at the absorption peak with S = 470 nm can be fitted to an approximate quadratic function:…”

“…One of the many excellent properties of metasurfaces is the efficient heat generation resulting from the localized surface plasmon resonance (LSPR) induced by light. As such, they are expected to find application in numerous fields, such as microfluidic control [5,6], nanoparticle capture [7][8][9], particle self-assembly [10], controlled drug release [11], polymerase chain reaction [12], tumor hyperthermia [13], bacterial killing [14], and photoacoustic imaging [15]. As a powerful tool in a broad range of applications, the miniaturization and integration of metasurfaces represent possible future directions for their development.…”

Integration of single‐cell transcriptome and proteome technologies: Toward spatial resolution levels

“…C Single-cell manipulation system using plasmonic tweezer. Reproduced with permission from Kang et al 31 Copyright 2023 John Wiley & Sons, Inc. D LCM utilizes microscopic visualization laser operation system to isolate cells from solid tissue samples. E FACS isolates single-cell by labeling cells with fluorescent marker proteins.…”

“…Lately, our group designed a plasmonic tweezer optical method (Figure 2C) consisting of an optical fiber probe, gold thin film, and thermosensitive hydrogel layer to realize selective capture of target cells, and it displays excellent 3D biocompatibility as well as low energy consumption. 31 Besides, the strategy of manual isolation namely laser capture microdissection (LCM) 34 (Figure 2D), can be available for collecting individual cells 35 or tissue regions of interest (ROI) from solid tissue samples 36,37 under microscopic visualization laser operation system. Laser-induced forward transfer (LIFT) 38 is a similar method to LCM, which has been successfully used to isolate single-cell 39,40 combined with other optical techniques.…”

Construction of multiplexed transcriptome NGS libraries of microdissected tissue samples based on combinational DNA barcode microbeads

“…In the last decade, the emergence and rapid development of single‐cell RNA sequencing (scRNA‐seq) has facilitated studies of cellular heterogeneity and has offered unprecedented insights into the mechanisms underlying diseases in individual organisms. [ 1–3 ] Single‐cell transcriptome profiles are obtained by combining single‐cell isolation techniques, such as microscopic manipulation, [ 4 ] fluorescence‐activated cell sorting (FACS), [ 5–6 ] or microfluidic approaches, [ 7–8 ] with compatible NGS library construction protocols, like full length transcript sequencing library preparation protocol, [ 9–11 ] 3′/5′ tag library construction technology, [ 12–13 ] whole transcript sequencing library method [ 14–15 ] and other microfluidic devices couple with previous workflow. [ 16 ] However, these dissociation‐based techniques lack spatial context, [ 17–18 ] which is essential for cellular bioanalysis.…”