Construction of multiplexed transcriptome NGS libraries of microdissected tissue samples based on combinational DNA barcode microbeads
Kaitong Dang,
Yue Zhao,
Kaiqiang Ye
et al.
Abstract:The combination of single‐cell RNA sequencing and microdissection techniques that preserves positional information has become a major tool for spatial transcriptome analyses. However, high costs and time requirements, especially for experiments at the single cell scale, make it challenging for this approach to meet the demand for increased throughput. Therefore, we proposed combinational DNA barcode (CDB)‐seq as a medium‐throughput, multiplexed approach combining Smart‐3SEQ and CDB magnetic microbeads for tran… Show more
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