A 39-kDa Capsular Protein is a Major Cross-Protection Factor as Demonstrated by Protection of Chickens with a Live Attenuated <i>Pasteurella multocida</i> Strain of P-1059

Abstract

ABSTRACT. The aim of this study was to show that a 39-kDa protein or OmpH of Pasteurella multocida strain P-1059 is essential for cross protection. Strain PBA322, a thinly capsulated strain of P. multocida strain P-1059, was used as a live vaccine in chickens. Strain PBA322 is a thinly capsulated strain in comparison with the parental strain P-1059. Chickens were vaccinated by single injection and then challengeexposed with strains P-1059 or X-73 at two weeks post vaccination. Moreover, immune responses were also evaluated for both humoral and cellular immune response by ELISA and lymphocyte proliferation assay, respectively. The results showed that the live vaccine induced efficient immunity to protect chickens from challenge-exposure to the parent strain, but that the heterologous protection was poor. We concluded that the 39-kDa protein is essential for cross protection.

Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H ofPasteurella multocidaSerovar A:1

“…In accordance with the previous study [ 8 ], the native form of OmpH induced an efficient antibody which inhibited the bacterium from adhering to CEF cells. Moreover, low amounts of OmpH in bacterial capsule affected the cross-protectivity [ 18 ]. However, the method requires further basic biochemistry coupled with bioinformatics tools to verify the protein structure.…”

Production of antibody against elephant endotheliotropic herpesvirus (EEHV) unveils tissue tropisms and routes of viral transmission in EEHV-infected Asian elephants

“…Rabbit anti-EEHV gB antibodies immunized with different concentrations of peptide-conjugated KLH carrier protein were subjected to either SDS-PAGE and stained with Coomassie Blue, as previously described 40 , or characterized for their specificity by western immunoblot analysis, immunohistochemistry, or immunofluorescence test, as described below.…”

miR‐155 promotes macrophage pyroptosis induced by Porphyromonas gingivalis through regulating the NLRP3 inflammasome

“…The NLRP3 inflammasome is activated by several factors, including ATP, sodium urate, cholesterol crystals, and bacteria. It is involved in the innate immune response and activates caspase-1, which cleaves and processes pro-IL-1β and pro-IL-18 into mature inflammatory cytokines (Nikaido, 2003;Prasadarao et al, 1996;Sthitmatee et al, 2013). IL-1β and IL-18 are the major factors secreted in an inflammatory response to periodontal infection (Belibasakis & Johansson, 2012).…”