A 3-O-methylated heterogalactan from Pleurotus eryngii activates macrophages

Yan, Jingmin; Yue, Meng; Zhang, Mengshan; Zhou, Xiaohuan; Cheng, Hairong; Sun, Lin; Zhou, Yifa

doi:10.1016/j.carbpol.2018.11.063

Cited by 37 publications

(15 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The column (OHpak SB-803HQ column, 8.0 × 300 mm, Shodex, Japan) was eluted with 0.2 M NaCl solution containing 0.02% NaN 3 at a flow rate of 0.6 ml/min at 35°C. A refractive index increment (dn/dc) of 0.185 ml/g was applied in this experiment ( Yan et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Jiao

Liu

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Two polysaccharides were obtained from steamed ginseng via ultrafiltration, and their physical–chemical properties, solution properties and antifatigue activities were studied. WSGP-S3 and WSGP-G3 were acid heteropolysaccharides with the molecular weights of 2.03 × 104 and 4.86 × 104, respectively. They were composed of different molar ratios of the monosaccharides Rha, GlcA, GalA, Glc, Gal, and Ara. The results of size-exclusion chromatography–multiangle laser light scattering analysis, Conge red staining and Circular dichroism spectroscopy revealed that WSGP-S3 exhibited a random conformation of branched clusters in solution. By contrast, WSGP-G3 exhibited an ordered conformation, including helix-like conformations in aqueous solution. Antifatigue activity tests proved that WSGP-S3 markedly prolonged the exhaustive swimming time of fatigued mice; increased liver and muscle glycogen levels and superoxide dismutase, catalase, glutathione peroxidase activities and decreased blood lactic acid, nitrogen and malondialdehyde levels compared with the control treatment. Moreover, it enhanced spleen cell proliferation in fatigued mice. By contrast, WSGP-G3 had no significant effect on fatigued mice. The results showed that WSGP-S3 might have a major contribution to the antifatigue effects of steamed ginseng polysaccharides and could be a potential anti-fatigue polysaccharide.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Jiao

Liu

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…In the research of Pleurotus eryngii polysaccharides, Xu et al found that a water-soluble polysaccharide EPA-1 could significantly induce macrophage to release the lever of NO, TNF-α, IL-1, and IL-6 through up-regulating signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κB [ 87 ]. Similarly, other studies also indicated that WPEP-N-b boosted the degradation of IκB-α, and increased phosphorylation of MAPKs and the NF-κB p65 subunit, which demonstrated this polysaccharide activates RAW264.7 cells through MAPK and NF-κB signaling pathways and the TLR2 [ 86 ]. In the research of Pleurotus florida polysaccharides, PS and glucan were found that could stimulate macrophages, splenocytes, and thymocytes.…”

Section: Antiviral Mechanisms Of Emfs Polysaccharidesmentioning

confidence: 82%

Classification, structure and mechanism of antiviral polysaccharides derived from edible and medicinal fungus

Guo

Chen

Gong

2021

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

The deficiency of chemical-synthesized antiviral drugs when applied in clinical therapy, such as drug resistance, and the lack of effective antiviral drugs to treat some newly emerging virus infections, such as COVID-19, promote the demand of novelty and safety anti-virus drug candidate from natural functional ingredient. Numerous studies have shown that some polysaccharides sourcing from edible and medicinal fungus (EMFs) exert direct or indirect anti-viral capacities. However, the internal connection of fungus type, polysaccharides structural characteristics, action mechanism was still unclear. Herein, our review focus on the two aspects, on the one hand, we discussed the type of anti-viral EMFs and the structural characteristics of polysaccharides to clarify the structure-activity relationship, on the other hand, the directly or indirectly antiviral mechanism of EMFs polysaccharides, including virus function suppression, immune-modulatory activity, anti-inflammatory activity, regulation of population balance of gut microbiota have been concluded to provide a comprehensive theory basis for better clinical utilization of EMFs polysaccharides as anti-viral agents.

show abstract

“…Overall, PPp-W was a 3-O-methylated heteroglycan and showed an irregular curly sheet with triple-helix conformation. It was reported that 3-O-Me-D-Galp was also found in P. eryngii, P. geesteranus, and P. pulmonarius (Smiderle et al, 2008;Zhang et al, 2013a;Zhang et al, 2013b;He et al, 2016;Brito et al, 2018;Yan et al, 2019). The summary of 3-O-Me -D-Gal p polysaccharides from Pleurotus spp.…”

Section: Glycosidic Linkage Analysismentioning

confidence: 95%

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

et al. 2022

View full text Add to dashboard Cite

Pleurotus placentodes, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of P. placentodes (PPp-W) and evaluate its anticoagulant activity in vitro. The high-performance liquid chromatography and GC–MS analysis indicated that PPp-W with a molecular weight of 27.4 kDa was mainly composed of mannose (17.56%), glucose (6.37%), galactose (44.89%), and fucose (1.22%) with a certain amount of 3-O-methyled galactose. SEM, XRD, and AFM combined with Congo red test revealed that PPp-W was an irregular curly sheet with triple-helix conformation. The FT-IR, methylation, and nuclear magnetic resonance analysis indicated that PPp-W contained→6)-α-D-Galp-(1→, →6)-3-O-Me-α-D-Galp-(1→and →2, 6)-α-D-Galp-(1→ as main chain, partially substituted at O-2 and O-6 by non-reducing ends of β-D-Manp-(1→ and β-L-Fucp-(1→ with a small amount of α-1,3-linked-Glcp in backbone. PPp-W could significantly prolong APTT (12.9 ± 0.42 s, p < 0.001) and thrombin time (39.9 ± 0.28 s, p < 0.01) compared with the control group (11.45 ± 0.071 s and 38.05 ± 0.21 s), which showed that PPp-W had anticoagulant activity. These studies suggested that PPp-W was a 3-O-methylated heteroglycan and might be suitable for functional foods and natural drugs as an anticoagulant ingredient, which provided a basis for the application of polysaccharides from P. placentodes.

show abstract

A 3-O-methylated heterogalactan from Pleurotus eryngii activates macrophages

Cited by 37 publications

References 43 publications

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Classification, structure and mechanism of antiviral polysaccharides derived from edible and medicinal fungus

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

Contact Info

Product

Resources

About