A 1H NMR based study of hemolymph metabonomics in different resistant silkworms, Bombyx mori (Lepidotera), after BmNPV inoculation

Wang, Xueyang; Shao, Zuo-min; Zhang, Yingjian; Vu, Thi Thuy; Wu, Yueming; Xu, Jia-ping; Deng, Mingjie

doi:10.1016/j.jinsphys.2019.103911

Cited by 21 publications

(24 citation statements)

References 43 publications

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“…The negative control is injected with BmN cell culture medium, and the blank control is given no injection. The resistant levels of YeA and YeB were tested in our previous report [ 2 ]. The medial lethal concentration (LC 50 ) value of YeA was more than 10 9 OB/mL, but p50 was just about 10 5 OB/mL.…”

Section: Resultsmentioning

confidence: 99%

“…BmNPV, one of the main silkworm pathogens, causes serious economic losses every year. However, the underlying molecular mechanism of silkworm in resistance to BmNPV infection remains unknown, even though many differentially resistant silkworm strains have been reported [ 2 , 25 , 26 ]. This suggests that there exists some kind of immune system in the silkworm organism, but this still needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…BmNPV is a double-stranded DNA virus that causes serious economic losses ever year. Many silkworm strains have been found to have a high resistance to BmNPV infection [ 1 , 2 ], but the molecular mechanism of silkworm resistance to BmNPV is still unclear. With the fast development of biotechnology, many new technologies have been used to study the antiviral mechanism of silkworms, such as the RNA-seq transcriptome [ 3 , 4 ], isobaric tag for relative and absolute quantification (iTRAQ), and label-free proteomics [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway

Wang

Ding

Chen

et al. 2020

Insects

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Discovery of the anti-BmNPV (Bombyx mori nuclearpolyhedrovirus) silkworm strain suggests that some kind of antiviral molecular mechanism does exist but is still unclear. Apoptosis, as an innate part of the immune system, plays an important role in the response against pathogen infections and may be involved in the anti-BmNPV infection. Several candidate genes involved in the mitochondrial apoptosis pathway were identified from our previous study. Bombyx mori apoptosis protease-activating factor-1 (Bmapaf-1) was one of them, but the antiviral mechanism is still unclear. In this study, sequences of BmApaf-1 were characterized. It was found to contain a unique transposase_1 functional domain and share high CARD and NB-ARC domains with other species. Relatively high expression levels of Bmapaf-1 were found at key moments of embryonic development, metamorphosis, and reproductive development. Further, the significant difference in expression of Bmapaf-1 in different tissues following virus infection indicated its close relationship with BmNPV, which was further validated by RNAi and overexpression in BmN cells. Briefly, infection of budded virus with enhanced green fluorescent protein (BV-EGFP) was significantly inhibited at 72 h after overexpression of Bmapaf-1, which was confirmed after knockdown of Bmapaf-1 with siRNA. Moreover, the downstream genes of Bmapaf-1, including Bmnedd2-like caspase (BmNc) and Bmcaspase-1 (Bmcas-1), were upregulated after overexpression of Bmapaf-1 in BmN cells, which was consistent with the RNAi results. Furthermore, the phenomenon of Bmapaf-1 in response to BmNPV infection was determined to be related to apoptosis using the apoptosis inducer NSC348884 and inhibitor Z-DEVD-FMK. Therefore, Bmapaf-1 is involved in the response against BmNPV infection by the mitochondrial apoptosis pathway. This result provides valuable data for clarifying the anti-BmNPV mechanism of silkworms and breeding of resistant silkworm strains.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway

Wang

Ding

Chen

et al. 2020

Insects

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“…To determine the response of Bmcytc to BmNPV, two different resistant silkworm strains AN and p50 were selected. The resistant levels of selected silkworm strain AN and p50 were determined in our previous study [16,17] the LC 50 value of AN was >10 9 OB/mL, p50 was about 10 5 OB/mL. The expression patterns of Bmcytc in the midgut, hemolymph, malpighian tubule and fat body of different resistant strains following BmNPV infection were analyzed using RT-qPCR (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)

Wang

Pang

et al. 2019

IJMS

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Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens of the silkworm. Cytochrome c (cytc) showed a significant response to BmNPV infection in our previous transcriptome study. However, little is known about the role of Bombyx mori cytc (Bmcytc) in resistance to BmNPV infection. In this study, the expression levels analysis of Bmcytc showed stable expression levels in selected tissues of the resistant strain AN following BmNPV infection, while there was downregulation in the susceptible strain p50, except in the malpighian tubule. To further study the role of Bmcytc in viral infection, Bmcytc was knocked down with siRNA in vitro, resulting in significant downregulation of selected downstream genes of the mitochondrial pathway, including Bmapaf, Bmcaspase-Nc, and Bmcaspase-1; this was also confirmed by overexpression of Bmcytc using the pIZT/V5-His-mCherry insect vector, except Bmcaspase-1. Moreover, knockdown of Bmcytc significantly promoted the infection process of BmNPV in vitro, while the infection was inhibited by overexpression of Bmcytc at the early stage and subsequently increased rapidly. Based on these results, we concluded that Bmcytc plays a vital role in BmNPV infection by regulating the mitochondrial apoptosis pathway. Our work provides valuable data for the clarification of the mechanism of silkworm resistance to BmNPV infection.

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“…A number of shellfish studies have been conducted to clarify the physiological roles of hemolymph serum proteins and hemocytes. A comparative 1 H NMR-based metabolomics was applied to analyze changes in the hemolymph metabolites of resistant silkworm strains after BmNPV inoculation [ 17 ]. The hemolymph-specific metabolite profiles in five Drosophila species before and after cold exposure were presented by Olsson et al [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

et al. 2020

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Metabolic profiling based on 1H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by 1H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from Helix aspersa. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry.

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A 1H NMR based study of hemolymph metabonomics in different resistant silkworms, Bombyx mori (Lepidotera), after BmNPV inoculation

Cited by 21 publications

References 43 publications

Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway

Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway

Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera)

An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

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