An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

Vassilev, Nikolay G.; Dangalov, Miroslav; Velkova, Lyudmila; Atanasov, Venceslav; Dolashki, Aleksandar; Dolashka, Pavlina

doi:10.3390/metabo10090360

Cited by 20 publications

(23 citation statements)

References 52 publications

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“…It is worth noting that observed results agree with the literature. In detail, H. aspersa mucus is a well‐known source of different amino acids, like proline and glycine found in different snail species, and sulfur‐bearing peptides 50 . Among these, glutathione (GSH) derivatives deserve a special attention, because their sulfhydryl group (−SH) belonging to cysteine units is involved in reduction and conjugation reactions for the removal of peroxides and many xenobiotic compounds, making them the major endogenous intracellular antioxidants 51 .…”

Section: Resultsmentioning

confidence: 99%

“…In detail, H. aspersa mucus is a well-known source of different amino acids, like proline and glycine found in different snail species, and sulfur-bearing peptides. 50 Among these, glutathione (GSH) derivatives deserve a special attention, because their sulfhydryl group (−SH) belonging to cysteine units is involved in reduction and conjugation reactions for the removal of peroxides and many xenobiotic compounds, making F I G U R E 1 MALDI (+) FT-ICR mass spectrum (A) and Van Krevelen diagram (B) obtained by the analysis of a sample of pure snail slime (SS). In plot B, types of formulae are distinguished by colors, that is, blue for CHO, green for CHON, red for CHONS, purple for CHOS, and yellow for CHN type them the major endogenous intracellular antioxidants.…”

Section: Maldi (+) Ft-icr Ms Analysismentioning

confidence: 99%

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Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Onzo

Pascale²,

Acquavia

et al. 2021

J Mass Spectrom

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Chronic wounds result from the failure of the normal wound healing process. Any delay during the tissue repair process could be defined as chronic wound healing, potentially having a highly detrimental impact on human health. To face this problem, in the last years, the use of different technologies alternative to therapeutic agents is gaining more attention. The Helix aspersa snail slime‐based products are increasingly being used for skin injury, thanks to their ability to make tissue repair processes faster. To date, a comprehensive overview of pure snail slime metabolome is not available. Besides, Au nanoparticles (AuNPs) technology is spreading rapidly in the medical environment, and the search for AuNPs “green” synthetic routes that involve natural products as precursor agents is demanded, alongside with a deep comprehension of the kind of species that actively take part in synthesis and product stabilization. The aim of this work is to characterize the metabolic profile of a pure snail slime sample, by an untargeted high‐resolution mass spectrometry‐based analysis. In addition, insights on AuNPs synthesis and stabilization by the main components of pure snail slime used to induce the synthesis were obtained. The untargeted analysis provided a large list of important classes of metabolites, that is, fatty acid derivatives, amino acids and peptides, carbohydrates and polyphenolic compounds that could be appreciated in both samples of slime, with and without AuNPs. Moreover, a direct comparison of the obtained results suggests that mostly nitrogen and sulfur‐bearing metabolites take part in the synthesis and stabilization of AuNPs.

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Section: Resultsmentioning

confidence: 99%

Section: Maldi (+) Ft-icr Ms Analysismentioning

confidence: 99%

Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Onzo

Pascale²,

Acquavia

et al. 2021

J Mass Spectrom

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“…Our previous studies described the antimicrobial activities of different fractions from the mucus of the garden snail C. aspersum, and characterized metabolites and antimicrobial peptides [34][35][36][37]. This study upgraded the previous data and provided additional information on new peptides and protein fractions with antibacterial activity in the mucus of C. aspersum.…”

Section: Introductionmentioning

confidence: 83%

Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of C. aspersum against Escherichia coli NBIMCC 8785

et al. 2022

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Peptides isolated from the mucus of Cornu aspersum could be prototypes for antibiotics against pathogenic bacteria. Information regarding the mechanisms, effective concentration, and methods of application is an important tool for therapeutic, financial, and ecological regulation and a holistic approach to medical treatment. A peptide fraction with MW < 10 kDa was analyzed by MALDI-TOF-TOF using Autoflex™ III. The strain Escherichia coli NBIMCC 8785 (18 h and 48 h culture) was used. The changes in bacterial structure and metabolic activity were investigated by SEM, fluorescent, and digital image analysis. This peptide fraction had high inhibitory effects in surface and deep inoculations of E. coli of 1990.00 and 136.13 mm2/mgPr/µMol, respectively, in the samples. Thus, it would be effective in the treatment of infections involving bacterial biofilms and homogenous cells. Various deformations of the bacteria and inhibition of its metabolism were discovered and illustrated. The data on the mechanisms of impact of the peptides permitted the formulation of an algorithm for the treatment of infections depending on the phase of their development. The decrease in the therapeutic concentrations will be more sparing to the environment and will lead to a decrease in the cost of the treatment.

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“…Bioactive compounds, such as proteins, peptides, and glycopeptides, are present in the mucus of many mollusks (Pitt et al, 2015). Recently, many compounds with antimicrobial and antioxidant activity, as well as regenerative properties were detected in the snail mucus (Dolashki et al, 2020; Pitt et al, 2019; Vassilev et al, 2020). Dolashki et al (2020) found that different peptide and protein fractions from the mucus of garden snail C. aspersum , manifest antibacterial activity against Gram negative ( Pseudomonas aureofaciens and Escherichia coli ) and Gram positive ( Brevibacillus laterosporus ) bacterial strains as well the anaerobic bacterium Clostridium perfringens (Dolashki et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity of garden snail mucus

et al. 2022

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The search for new natural compounds for application in medicine and cosmetics is a trend in biotechnology. One of the sources of such active compounds is the snail mucus. Snail physiology and the biological activity of their fluids (especially the mucus) are still poorly studied. Only a few previous studies explored the relationship between snails and their microbiome. The present study was focused on the biodiversity of the snail mucus used in the creation of cosmetic products, therapeutics, and nutraceuticals. The commonly used cultivation techniques were applied for the determination of the number of major bacterial groups. Fluorescence in situ hybridization for key taxa was performed. The obtained images were subjected to digital image analysis. Sequencing of the 16S rRNA gene was also done. The results showed that the mucus harbors a rich bacterial community (10.78 × 1010 CFU/ml). Among the dominant bacteria, some are known for their ability to metabolize complex polysaccharides or are usually found in soil and plants (Rhizobiaceae, Shewanella, Pedobacter, Acinetobacter, Alcaligenes). The obtained data demonstrated that the snail mucus creates a unique environment for the development of the microbial community that differs from other parts of the animal and which resulted from the combined contribution of the microbiomes derived from the soil, plants, and the snails.

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An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

Cited by 20 publications

References 52 publications

Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Untargeted analysis of pure snail slime and snail slime‐induced Au nanoparticles metabolome with MALDI FT‐ICR MS

Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of C. aspersum against Escherichia coli NBIMCC 8785

Microbial diversity of garden snail mucus

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