A 14C- and 3H-thymidine double labeling technique in the study of cell proliferation in Tradescantia root tips

Wimber, Donald E.; Quastler, Henry

doi:10.1016/0014-4827(63)90209-x

Cited by 212 publications

(58 citation statements)

References 6 publications

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“…That irradiation prolongs the mitotic cycle duration (DREW and PAINTER 1959) and more particularly the G. period (CHEVREMONT-COMHAIRE et al 1962;WIMBER and QuASTLER 1963;BASSLEER and CH:EvREMONTCoMHAIRE 1964 ). In our first experiment, bridges and fragments were detected at anaphase.…”

Section: Estimation Of G1mentioning

confidence: 99%

Duration of Mitotic Cycle and Patterns of DNA Replication in Chromosomes ofAllium Cepa

Matagne

1968

Caryologia

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Section: Estimation Of G1mentioning

confidence: 99%

Duration of Mitotic Cycle and Patterns of DNA Replication in Chromosomes ofAllium Cepa

Matagne

1968

Caryologia

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“…Another procedure to estimate S-phase duration consists in the applica tion of 2 successive pulse labels, the 1st with 3H-thymidine, and the 2nd, after a period equal to or less than G2, with C14-thymidine [38,69]. The cells labelled with 3H alone and those labelled with C14 can be distinguished by using special autoradiographic procedures.…”

Section: Duration O F the Dna Synthetic Phasementioning

confidence: 99%

“…3). The duration of S (Ts) may be calculated from the equation [69] N h _ At Nc ~ Ts where N h represents the number of cells labelled with 3H only and Nc the number of cells containing 14C.…”

Section: Duration O F the Dna Synthetic Phasementioning

confidence: 99%

Cell Renewal in the Gastric Mucosa

Willems¹

1972

Digestion

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The mechanisms controlling cell division in the stomach are still unknown. Among the procedures available for determining kinetic parameters in the mucosa, autoradiographic methods with ³H-thymidine are the most widely used. As yet, they have allowed observation of two basic control factors in the gastric mucosa: A tissue specific inhibitory substance of mitotic activity called ‘gastric chalone’ and a stimulating hormone released after food ingestion, which is most probably gastrin. Other possible factors are now to be further investigated.

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“…It is based on quantifying the proportion of cells exiting or entering S-phase during the interval between labels and thus containing only one label rather than two (Wimber & Quastler, 1963). Double labelling can also be performed using two different radioisotopes to label thymidine, which can then be deteced in different layers of autographic emulsion (Schultzê , 1976).…”

Section: Can T Of Explants Be Measured In Vitro?mentioning

confidence: 99%

Cell kinetic analysis of murine squamous cell carcinomas: a comparison of single versus double labelling using flow cytometry and immunohistochemistry

Schultz-Hector¹,

Begg²,

Hofland³

et al. 1993

Br J Cancer

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Summary The study was originally set up to measure accurate cell kinetic parameters in two murine squamous cell carcinomas (scc) for comparison with radiobiological data on proliferation during radiotherapy. The tumours, AT84 and AT478, were both moderately well differentiated aneuploid scc. In the course of the study, several Tumour cell kinetic studies are not only essential in investigating the many factors regulating proliferation but have also been shown to be useful for predicting the response to therapy (Silvestrini et al., 1984;Tubiana et al., 1989;Begg et al., 1992;Begg, 1993). Most cell kinetic studies have been carried out using either radio-labelled thymidine or a thymidine analog, which are incorporated into cells undergoing DNA synthesis. Such labelling of S-phase cells can be carried out in vivo or in vitro. In patients, administration of radiolabelled DNA precursors is precluded because of radiation hazards. Consequently, clinical studies have employed labelling of biopsy material in vitro (Silvestrini et al., 1984;Tubiana et al., 1989; The original goal of these studies was to measure accurately the cell kinetic parameters in two murine tumours for correlations with radiobiological characteristics of tumour proliferation during radiotherapy . The radiobiological data were available for tumours at two sizes, necessitating kinetic measurements at both these sizes. It was planned to use both FCM and IHC methods to ensure an accurate and correct description of the cell kinetics. The study was then also used to attempt to answer the questions posed above in these two well described animal tumour models. It thus provides comparisons of FCM and IHC methods, in vitro with in vivo labelling, single vs double labelling, the magnitude of intratumoural variations in kinetic parameters, in addition to the influence of tumour size. Materials and methodsTumours and tumour growth rate AT84 and 478 are both fairly well differentiated murine squamous cell carcinoma lines, derived from spontaneous tumours of the oral and vaginal mucosa respectively. An early, more slowly growing (AT478/4) and a late, much faster (AT478/25) generation of one tumour line were compared.

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A 14C- and 3H-thymidine double labeling technique in the study of cell proliferation in Tradescantia root tips

Cited by 212 publications

References 6 publications

Duration of Mitotic Cycle and Patterns of DNA Replication in Chromosomes ofAllium Cepa

Duration of Mitotic Cycle and Patterns of DNA Replication in Chromosomes ofAllium Cepa

Cell Renewal in the Gastric Mucosa

Cell kinetic analysis of murine squamous cell carcinomas: a comparison of single versus double labelling using flow cytometry and immunohistochemistry

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