A 12-month longitudinal study of Listeria monocytogenes contamination and persistence in pork retail markets in China

Luo, Lijuan; Zhang, Zhengdong; Wang, Hong; Wang, Pengfei; Lan, Ruiting; Deng, Jianping; Miao, Yimao; Wang, Yan; Wang, Yi; Xu, Jianguo; Zhang, Ling; Sun, Songsong; Liu, Xiang; Zhou, Yang; Chen, Xi; Li, Qun; Ye, Changyun

doi:10.1016/j.foodcont.2016.12.037

Cited by 26 publications

(26 citation statements)

References 37 publications

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“…The predominant L. monocytogenes subtype strains (1/2c/PT4/ST9) isolated from environment (including MCS and NMCS) and insects were also the main subtype strains existed in raw pork. We also found that prevalence of L. monocytogenes was higher in months with higher temperature in our study, which was different from the patterns of report about that in southwest China ( Luo et al, 2017 ), which possibly attributed to the difference of regional climate and retail environment.…”

Section: Discussioncontrasting

confidence: 99%

“…In this study, the L. monocytogenes contamination rate in raw pork was 29%, which is higher than that of previous reports in China and other countries such as Chile and Japan (6–21%) ( Inoue et al, 2000 ; Chen et al, 2015 ; Montero et al, 2015 ; Wang K. et al, 2015 ; Wang W. et al, 2015 ; Luo et al, 2017 ). This higher rate of L. monocytogenes contamination in raw pork was more likely due to the contamination in the retail market rather than upstream of the processing or retail chain, because our previous study showed that L. monocytogenes contamination in slaughterhouses in Beijing was very low (0.5%) ( Liu et al, 2014 ).…”

Section: Discussioncontrasting

confidence: 70%

“…It can adhere to many surfaces and form biofilms, and has the ability to survive and grow at low temperatures, a wide range of pH, high salt concentrations and low water activity ( Duffy and Sheridan, 1997 ; Miettinen et al, 1999a , b ; Freitag et al, 2009 ). L. monocytogenes has been isolated from various food products, including raw and cooked meats, milk products, seafood and vegetables ( Ballesteros et al, 2011 ; Jami et al, 2014 ; Moosavy et al, 2014 ; Brown et al, 2016 ; Luo et al, 2017 ). High levels of L. monocytogenes contamination in raw pork in China have been reported ( Zhang et al, 2007 , 2013 ; Yang et al, 2008 ; Huo et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the understanding of risk factors of L. monocytogenes contamination of raw meat in retail market is limited, especially in China. A recent study showed that the retail environment played an important role in L. monocytogenes contamination in pork retail markets, but few environmental samples were collected ( Luo et al, 2017 ). Insects such as flies and cockroaches can carry pathogens ( Barreiro et al, 2013 ), but little is known about their role in L. monocytogenes transmission in China.…”

Section: Introductionmentioning

confidence: 99%

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Risk Factors and Level of Listeria monocytogenes Contamination of Raw Pork in Retail Markets in China

Wang

Lan

et al. 2018

Front. Microbiol.

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Listeria monocytogenes can contaminate various foods via food processing environments and contamination of raw materials. There is a limited understanding of L. monocytogenes transmission in retail market and the role of insects in L. monocytogenes transmission in the retail environments. To better understand the risk factors of raw pork contamination, the prevalence of L. monocytogenes was examined in raw pork, retail environments and insects in a retail market over a 6-month period from March to August in 2016 in Beijing, China. A total of 2,789 samples were collected, including 356 raw pork samples, 1,392 meat contact surface swabs (MCS), 712 non-meat contact surface swabs (NMCS) and 329 insect samples. Overall, 424 (15.20%) of the samples were found to be contaminated by L. monocytogenes. Analyzed by serotyping, multilocus sequence typing and pulsed-field gel electrophoresis, the 424 L. monocytogenes isolates were divided into three serotypes (1/2c, 1/2a and 3a), 15 pulsotypes (PTs) and nine sequence types (STs), 1/2c/PT4/ST9 (244/424, 58%) was the most prevalent type of L. monocytogenes strains. The raw pork, MCS of the environments and insects were contaminated with higher levels of L. monocytogenes than NMCS of the environments, which suggested that cross contamination of L. monocytogenes between raw pork and the environment existed in the retail market, and long-term contaminated surfaces and vector insects would act as high risk factors to transmit L. monocytogenes to raw pork. Thus more effective strategies are needed to reduce the risk of retail pork meat contamination by L. monocytogenes and prevent foodborne human listeriosis.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Risk Factors and Level of Listeria monocytogenes Contamination of Raw Pork in Retail Markets in China

Wang

Lan

et al. 2018

Front. Microbiol.

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“…L. monocytogenes also can adhere to the surfaces of food processing equipment and resist adverse conditions. It has been reported that L. monocytogenes was persistently isolated from raw pork in open markets in China (Luo et al, 2017 ). L. monocytogenes is aerobic, or facultatively anaerobic under some conditions.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of als Genes Involved in D-Allose Metabolism in Lineage II Strain of Listeria monocytogenes

et al. 2018

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Listeria monocytogenes, an important food-borne pathogen, causes listeriosis and is widely distributed in many different environments. In a previous study, we developed a novel enrichment broth containing D-allose that allows better isolation of L. monocytogenes from samples. However, the mechanism of D-allose utilization by L. monocytogenes remains unclear. In the present study, we determined the metabolism of D-allose in L. monocytogenes and found that lineage II strains of L. monocytogenes can utilize D-allose as the sole carbon source for growth, but lineage I and III strains cannot. Transcriptome analysis and sequence alignment identified six genes (lmo0734 to 0739) possibly related to D-allose metabolism that are only present in the genomes of lineage II strains. Recombinant strain ICDC-LM188 containing these genes showed utilization of D-allose by growth assays and Biolog phenotype microarrays. Moreover, lmo0734 to 0736 were verified to be essential for D-allose metabolism, lmo0737 and 0738 affected the growth rate of L. monocytogenes in D-allose medium, while lmo0739 was dispensable in the metabolism of D-allose in L. monocytogenes. This is the first study to identify the genes related to D-allose metabolism in L. monocytogenes, and their distribution in lineage II strains. Our study preliminarily determined the effects of these genes on the growth of L. monocytogenes, which will benefit the isolation and epidemiological research of L. monocytogenes.

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Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases

et al. 2018

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This study aimed to investigate the prevalence and molecular characteristics of Listeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat (RTE) shops and mutton restaurants surveyed was 16.2% (141/873). An occurrence of 13.5% was observed in RTE pork, 6.5% in RTE vegetables, and more than 24.0% in either cooked mutton or cooked haggis. Serotype 1/2b (45.4%), 1/2a (33.3%), and 1/2c (14.2%) were the predominant types. By comparing the clonal complexes (CCs) based on multilocus sequence typing (MLST) of the L. monocytogenes from cooked foods in Zigong City and 33 listeriosis cases from different districts of China, CC87, CC9, CC8, and CC3 were showed to be prevalent in cooked products and CC87 and CC3 were the first two frequent types in the 33 clinic-source strains. All CC87 stains harbored the newly reported Listeria pathogenicity island 4 (LIPI-4) gene fragment ptsA, and all CC3 strains possessed the Listeria pathogenicity island 3 (LIPI-3) gene fragment llsX. These may increase the occurrence of the strains belonging to CC87 and CC3 in listeriosis cases in China and also underline the risk of infection owing to the consumption of the cooked products from Zigong. ST619 (serotype 1/2b) harbored both llsX and ptsA, indicating a potential hypervirulent sequence type in Zigong.

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A 12-month longitudinal study of Listeria monocytogenes contamination and persistence in pork retail markets in China

Cited by 26 publications

References 37 publications

Risk Factors and Level of Listeria monocytogenes Contamination of Raw Pork in Retail Markets in China

Risk Factors and Level of Listeria monocytogenes Contamination of Raw Pork in Retail Markets in China

Identification and Characterization of als Genes Involved in D-Allose Metabolism in Lineage II Strain of Listeria monocytogenes

Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases

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