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Abstract

HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is transient in nature. In the temperature regime tested, 45 degrees C treatment to intact rice seedlings for 2 h showed maximal levels of hsp101 mRNA. Rice full-length hsp101 cDNA complemented yeast mutant disrupted for its own hsp104 gene by insertional mutagenesis, with efficacy that was comparable with Arabidopsis hsp101 cDNA. Electron micrographic evidence suggested that rice hsp101 cDNA in yeast is active in re-solubilizing the stress-induced protein granules in the post-stress recovery period. Rice hsp101 cDNA expression in hsp104 deficient yeast also caused recovery in tolerance against arsenite. Western analyses showed that this protein is expressed more rapidly during the stress period and retained for longer duration in the post-stress recovery period in japonica rice as compared to indica rice types. This is the first report wherein plant HSP100 protein expression is correlated to disappearance of protein granules in the yeast cells and distinct rice type-dependent protein expression patterns are reported.

Stress Response and Tolerance of Zea mays to CeO2 Nanoparticles: Cross Talk among H2O2, Heat Shock Protein, and Lipid Peroxidation

“…In addition, it was noted that even on day 20, at a time when the over produced H 2 O 2 had been eliminated by the CAT and APX, the production of HSP70 was still higher compared to control. Agarwal et al 27 showed that, in rice, HSP101 was produced during the post-stress phase, implying its role in the plant’s recovery to normal conditions after stress. Our data suggest that the HSP70 protects the plants from potential damage, which might be caused by excessive deposition of NPs/NP aggregates.…”

Interaction Studies of Heat Shock Responsive Protein HSA32 and Heat Shock Protein HSP101: An In Silico Approach

“…It is also suggested that HSA32 complements HSP101 when plants are exposed to high temperature and activates theromotolerance mechanism. Heat induced HSP101 decayed differentially in indica and japonica rice [11]. It suggests a role of regulating HSP101 decay in the adaptation to changing climates.…”

Transcriptional Profiling and Identification of Heat-Responsive Genes in Perennial Ryegrass by RNA-Sequencing

“…Based on their approximate molecular weight, the principal HSPs are grouped into six conserved classes: HSP100/Clp, HSP90/HtpG, HSP70/DnaK, HSP60/GroEl, HSP40/DnaJ, and the small heat shock proteins (sHSPs) (Bharti and Nover, 2002; Schulze et al, 2005). The up-regulation of this gene group is well documented when cells are exposed to elevated temperatures or other stresses (Ada et al, 1987; Lee et al, 2000; Agarwal et al, 2003; Kant et al, 2008). It is therefore not surprising that the expression of many HSPs increased after exposure to heat stress in the study.…”