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Abstract

Biofilm are a matrix of microorganisms which are adhered to and colonized a surface. When formed they are very difficult to remove and act as a source of contamination in processing environments. As bacteria in biofilm exhibit enhanced resistance to antibiotics and clearance by the host immune system, the resistance of enteropathogenic bacteria to commonly prescribed antibiotics is increasing both in developing as well as in developed countries. Resistances have emerged even to newer, more potent antimicrobial agents. This study was under taken to investigate the presence of multidrug resistance producing biofilm forming Aeromonas hydrophila in human clinical samples. A total of 150 urine samples were collected from private hospital in Tiruchengode during the period of six month. Among these only 75 isolates were found to be positive for Aeromonas hydrophila. The Starch-Ampicillin agar were used as a selective presumptive isolation medium for the isolation of bacterial isolates and confirmed as Aeromonas hydrophila were determined by using standard biochemical analysis according to Bergey's manual of systematic Bacteriology. Slime producing isolates were studied on Congo Red Agar (CRA) plate method and the biofilm were determined in tube method. Multi-drug resistance patterence and MDR index were carried out according to the criteria of national committee for clinical laboratory standards. Infection due to bacterial pathogen with such virulent factors (biofilm) act as a one of the source for multi-drug resistance producing isolates among the microbial population. Aeromonas hydrophila has received particular attention because of its association with human infection. So that, in this present study the slime and biofilm forming isolates was detected and studied their multi-drug resistance patterns. Urine samples were collected from private hospital in Tiruchengode was found to contain very diverse populations of biofilm forming Aeromonas hydrophila.