The misuse and overuse of antibiotics within poultry sector of the animal agriculture threatens public health due to its aftermath effect arising from the release of resistant pathogens to the environment. This study investigated the prevalence and antimicrobial susceptibility status of bacterial species isolated from litter of selected poultry farms within Idi Ayunre, a poultry-dense community in Oyo State. Poultry droppings (5 g) was collected each from six (6) randomly selected farms and bacteria isolated using Brain Heart Infusion agar (BHI), MacConkey agar (MCA), Eosin methylene blue agar (EMB) and Salmonella Shigella agar (SSA) media respectively via pour plate technique. Bacterial isolates were identified via morphological and biochemical tests and their antibiotic susceptibility pattern determined via Kirby-Bauer disc diffusion method using multi-disc antibiotics. Farm A had highest Total Heterotrophic Count (THC, 8.43 Log10CFU/mL) and Total Enterobacteriaceae Count (TEC, 6.32 Log10CFU/mL). However, Farm C had lowest THC and TEC (7.18 Log10CFU/mL and 4.48 Log10CFU/mL respectively), while Farm B had lowest Total Coliform Count (TCC, 4.70 Log10CFU/mL) with the highest being Farm D (6.08 Log10CFU/mL). Also Farm D had the highest (8.15 Log10CFU/mL) Salmonella-Shigella Count (SSC) with Farm E having the lowest (4.85 Log10CFU/mL). Isolates obtained were identified as Staphylococcus aureus, Proteus sp., Bacillus sp., Pseudomonas sp, Klebsiella sp., Escherichia coli, Citrobacter sp., Salmonella sp., Enterobacter sp. and Shigella sp. Across the farms, S. aureus, Bacillus sp. and E. coli were isolated, while Shigella sp. was isolated only from Farm D. The bacteria isolates were found to be multidrug resistant towards the tested antibiotics, showing susceptibility only to gentamicin and ofloxacin. This study therefore showed that birds’ droppings from poultry farms in Idi Ayunre community serve as carrier of multidrug resistant pathogens. Hence, the need for a cautious use of antibiotics among Idi Ayunre poultry farmers.

This study assessed the low-density polythene (LDPE) lm degradation potential of microorganisms isolated from oil contaminated soil, and characterizing the LDPE lms after degradation period by analyzing the morphological and chemical composition. The bacteria strains isolated from petroleum contaminated soils were used as bio-degraders for the analysis and isolates with great degradation activity were identi ed via 16S RNA gene ampli cation technique. The growth rates of the bacterial isolates and their LDPE utilization in the medium were monitored for 78 days, using UV spectrophotometry. Thereafter, the LDPE in the media were quantitatively and qualitatively analysed. Results obtained from weight loss measurement, scanning electron microscope (SEM) images and energy dispersal x-ray (EDX) revealed a reduction in weight, porosity and fragility of all the analyzed LDPE lms that were subjected to bacteria degradation. Isolates with good degradation of LDPE lms were identi ed as Proteus mirabilis (Accession number: MN124173.1) and Proteus mirabilis (Accession number: KY027145.1), having a weight loss of 89.72% and 71.80% respectively. The identi cation and utilization of potential LDPE microbial degraders are useful in the management of plastic waste, leading to a reduction in global plastic waste and a clean environment.