Untitled

Abstract

The development of new materials to be used as a substrate for plant tissue culture can lead to substantial advances in biotechnology. Here, mats consisting of a mixture of nonwoven (randomly oriented) and aligned nanofibres were produced by electrospinning solutions of polylactic acid (PLA) and polyvinylidene fluoride (PVDF). They were referred to as PLA4 and PVDF4, respectively. Callus initiation from stem explants of bilimbi (Averrhoa bilimbi L.) and aromatic chilli (Capsicum frutescens L.) occurred on these two types of nanofibre mats floated in liquid Murashige and Skoog (1962) basal medium supplemented with 2 mg/l α-naphthalene acetic acid or 2,4-dichlorophenoxy acetic acid, respectively. After subculturing for 3 weeks, the fresh weight of callus initiated from stem explants of bilimbi was significantly greater by 20% when PVDF4 rather than PLA4 nanofibre mats were used as a support matrix. In contrast, both the fresh and dry weights of callus initiated from stem explants of aromatic chilli were significantly greater by 11% and 50%, respectively, when PLA4 and not PVDF4 nanofibre mats was used. These differences in response to the two types of nanofibre mats were also found in relation to growth of callus of bilimbi and aromatic chilli during subculture. This is the first time electrospun nanofibre mats have been used in plant tissue culture research.

Growth and root formation in the microshoots of three basils cultured in vitro on different types and patterns of electrospun nanofibre mats

“…Electrospun nanofibre mats were first used in plant tissue culture for callus induction and subculture (Bodhipadma et al, 2011) and later for pollen germination (Bodhipadma et al, 2016). In both studies, there were also different growth responses on the diverse types and patterns of nanofibre mats as found in the present research.…”

“…An electrospun nanofibre mat is a physical support matrix containing tens to hundreds of nanometer or sub-nanometer fibres which were electrospun and conventionally fabricated into mats with the fibre in random, aligned or patterned arrangements (Bodhipadma et al, 2016;Mahjour et al, 2016;Jian et al, 2018;Kozior et al, 2019). These electrospun ultrafine fibres could be produced from inorganic or organic compounds, for example, polylactic acid, -2 -polyvinylidene fluoride (Bodhipadma et al, 2011), polycaprolactone (Chanunpanich & Suwanboon, 2014), fish skin gelatin (Songchotikunpan et al, 2008) and lignin (Hong et al, 2019). So far, there were various applications of electrospun nanofibre mats, including filtrations, affinity membranes and recovery of metal ions, tissue engineering scaffolds, wound healing, drug delivery, composites and templates, catalyst and enzyme carriers, sensors and energy storage (Jian et al, 2008;Wang & Ryan, 2011).…”

“…In plant tissue culture, electrospun nanofibre mats have recently been applied in a few experiments, for instance, as a scaffold for callus induction and culture of aromatic chilli and bilimbi (Bodhipadma et al, 2011) and a physical support substrate for germination of the Artabotrys hexapetalus pollen (Bodhipadma et al, 2016). A microshoot is an explant comprising the apical meristem with a minimal amount of other shoot tissues.…”