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Abstract

We developed and described a new approach to vital analysis of functional activity of multidrug resistance markers (ABC transporters) in intact biopsy specimens from human solid tumors by the method of flow cytofluorometry. The algorithm of the study underwent revision, and the cell suspension was obtained in the final stage. Intensification of intracellular doxorubicin accumulation (fluorescence) and increase in the number of fluorescent cells and total fluorescence of doxorubicin-accumulating cells produced by ABC transporter inhibitor sodium azide served as the criteria of expression of these transporters in tumor tissue. Informative value of changes in various parameters of doxorubicin fluorescence is discussed. The increase in the count of fluorescent cells in the suspension of tumors cells after treatment with the inhibitor indicates the presence of tumor cells absolutely resistant to this preparation. The proposed method is technically simple, suitable for structurally different tumors, requires small amounts of the biopsy material and, therefore, can be used for routine analysis. The results of our analysis and spectrofluorometric assay of ABC transporters agree very closely, which suggest that this method is adequate for the purpose.

Feasibility Investigation and Characterization of Liquid Dispersant–Assisted Sintering of Silver to Bond Large‐Area Plates

“…[36,37] It is found that PEG can reduce the surface tension, prevent silver volume nucleation, and help to fill the narrow trenches. [38] German et al [39] mentioned that liquid can not only generate a rearrangement torque to bring flat surface into contact, but also induce a capillary force to accelerate diffusion and rearrangement of metal particles. Fouad et al [40] employed PEG as a main reducing agent and PVP as a capping and dispersing agent to synthesize metal conductive nanopigments.…”

A New Approach to the Estimation of Functional Activity of ABC-Transporters Regulating Intracellular Distribution of Anticancer Drugs Using Flow Cytometry

“…Functional activity of ABCtransporters was estimated by flow cytometry procedure adapted for studying solid tissues [6].…”