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Abstract

Angiogenesis is essential in wound healing and a common feature in chronic inflammation which is crucially involved in the biological response to biomaterials. A useful system to evaluate the angiogenic activity and the inflammatory potency of various agents is the chorioallantoic membrane (CAM) of the chick embryo. Here we examined its response to different biomaterials. Smooth materials such as PVC or the polyurethane Tecoflex either unmodified or modified by an OH- or N(CH(3))(3)(+)-end group (HEMA or MAPTAC) inhibited angiogenesis and did not induce the formation of granulation tissue. The anti-angiogenic effects of PVC, Tecoflex and its HEMA modification, however, were only seen at an early stage of development. In contrast, the MAPTAC modified Tecoflex inhibited angiogenesis over the whole time. Rough materials, e.g. filter paper or a collagen/elastin membrane, stimulated angiogenesis and induced the formation of inflammatory tissue. Histological analysis revealed that the filter material was homogeneously populated with cells consisiting mainly of macrophages, fibroblasts and endothelial cells. The collagen/elastin membrane was only partially infiltrated with cells. Among those also clusters of granulocytes were present pointing to an acute inflammatory process. These data show that the angiogenic activity and inflammatory response of biomaterials strongly depend on the chemical composition and the physical structure of the material. The CAM assay appears to be a useful tool for studying biocompatibility.

Chitosan–aluminum monostearate composite sponge dressing containing asiaticoside for wound healing and angiogenesis promotion in chronic wound

“…This study was approved by the Animal Ethics Committee, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand. In ovo method was performed to investigate the angiogenic activity of asiaticoside using the method described previously [23] with some modifications. Fertilized chicken eggs were incubated in a humidified incubator at 37°C with adjusting slope of the egg tray back and forth for 60°every 2 h.…”

The RGD Coupling Strategy Determines the Inflammatory Response of Human Primary Macrophages In Vitro and Angiogenesis In Vivo

“…We found that covalent coupling of the peptide using the pAAc strategy resulted in a significant decrease in angiogenesis after seven days, exhibiting the lowest number of novel blood vessels, but strikingly, it increased up to the highest level of angiogenesis of all materials tested in this study in as few as two additional days (Table ). As another readout of the CAM assay, granulation tissue can be determined . Notably, only the covalently coupled peptide also evoked the development of inflammatory granulation tissue (indicated by arrows) after nine days of the assay (Figure ).…”

“…The opening in the shell was sealed with paraffin film (Parafilm, Pechiney Plastic Packaging, Chicago, USA), to prevent dehydration. The eggs were incubated at 37 °C for another 7 or 9 d as described earlier …”

“…The chorioallantoic membrane (CAM) of the Gallus gallus domesticus (chicken) embryo is an established, cost‐efficient in vivo model for development, angiogenesis, surgery, and biomaterials research . The CAM of chicken starts to form seven days after incubation out of chorion and allantois and represents a matrix for the developing vascular system .…”

BIOARTIFICIAL POLYMER MATERIALS BASED ON PVC/NATURAL POLYMER BLENDS: BINARY PVC/HYDROLYZED COLLAGEN BLENDSv

“…Usually, the polyvinyl chloride (PVC) films satisfy some requirements imposed by medical applications, such as chemical inertness, biocompatibility, ease of design and lower cost of the existing technologies [7,8]. An increased concentration of plasticizer significantly reduces the yellowness index of materials after gamma sterilization [9].…”