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Abstract

The present study is designed to evaluate the impact of potential probiotics Enterococcus faecium QAUEF01 in single and its mix-culture with Geotrichum candidum QAUGC01 on the Labeo rohita (Hamilton, 1822). In the mix-culture, both bacteria and yeast survived comparatively better under mimic gut conditions and showed higher hydrophobicity. Moreover, mix-culture showed comparatively more antipathogenic activity. A feeding trial of 90 days for L. rohita fingerlings comprising of three treatments, control group fed on basal diet, second group fed on E. faecium supplemented diet and third group was fed on mix-culture probiotics supplemented diet. Mix-culture probiotics fed group showed significantly higher (P<0.05) growth as compared to control. Better specific growth rate (SGR) was significantly correlated with the feed conversion ratio (FCR) and feed conversion efficiency (FCE), protease and cellulase activity in probiotic fed fishes. The applied probiotics established well in fish gut and shown no harmful impact on fish physiology. Probiotic application distinctly modulated fish gut microbiome evident by increased level of friendly microbiota and exclusion of potential fish pathogens. The results suggested an effective eco-friendly strategy to boost the fish productivity.

Evaluation of yeast and bacterial based probiotics for early rearing of Labeo rohita (Hamilton, 1822)

“…The result of the study is in agreement with Ibrar et al (2017) findings. It seems that G. candidum due to their higher enzymatic potential, improves the digestibility and absorption of nutrients more efficiently (Piegza et al, 2014), thus, increasing the status of fat and protein contents in the muscle.…”

“…No comparable study is available to reveal the antagonist activity of G. candidum against A. hydrophila in fish. However, in vivo antagonist activity against Staphylococcus aureus (Ibrar et al, 2017), as well as in vitro antimicrobial activity alone (Naz, Gueguen-Minerbe, Cretenet, & Vernoux, 2013;Samuel, Prince, & Prabakaran, 2017) or in mix culture with Enterococcus faecium (Ghori et al, 2018) Overall results of the present study showed that G. candidum-QAUGC01 alone has great potential to improve survival, growth rate, proximate composition of muscle and resistance to pathogen during early rearing of L. rohita. These positive effects may be ascribed to their morphological structure, higher enzymatic potential and immune-stimulatory effect.…”

“…Previously, Ibrar et al (2017) also observed beneficial effect of similar strain of G. candidum as water additive on early rearing of rohu. However, here the observed weight gain is several folds higher than reported in earlier study.…”

“…It seems that way of administration of probiotic and feeding strategy affect the results of rearing (De La, 2001). We changed the feeding rate, frequency as well feed particle size of feed fortnightly (Table 2) and observed most significant effect on growth and survival of swim up fry of L. rohita even in control group as compared to other studies (Ibrar et al, 2017;Jha et al, 2015), where L. rohita spawn were reared on fixed feeding frequency, ration and particles size. (Ghosh et al, 2002) also support our results.…”

“…To determine amylase activity, 3,5-dinitrosalicylic acid (DNS) method reported previously (Ibrar et al, 2017;Ullah et al, 2018) was adopted. The method generally based on estimation of glucose (reducing sugar) at 560 nm.…”