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Malykh, E V; Tiourina, O. P.; Ларионова, Н. И.

doi:10.1023/a:1010209715005

Cited by 9 publications

(1 citation statement)

References 19 publications

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“…Amphiphilic poly-N-vinylpyrrolidones were synthesized in two steps, similar to our previous experiments [19,20]. The BBI, modified by one (Ole 1 -BBI) or two (Ole 2 -BBI) oleic acid residues, was prepared using previously developed methods [27][28][29]. The investigation of amphiphilic PVP self-assembling processes in the absence and in the presence of BBI preparations was carried out by addition of appropriate amounts of protein to the PVP-OD solutions with different concentrations in distilled water or in a physiological solution (0.15 NaCl, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

Kuskov¹,

Villemson²,

Штильман³

et al. 2007

J. Phys.: Condens. Matter

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New nanoscaled polymeric carriers have been prepared on the basis of different amphiphilic water-soluble derivatives of poly-N-vinylpyrrolidone (PVP). The polymer self-assembly and interaction with model proteins (Bowman-Birk soybean proteinase inhibitor (BBI) and its hydrophobized derivatives) were studied in aqueous media. The possibility of inclusion of both BBI and hydrophobized oleic acid derivatives of BBI in amphiphilic PVP aggregates was investigated. It was ascertained that polymeric particles of size 50-80 nm were formed in certain concentrations of amphiphilic PVP and poorly soluble dioleic acid derivatives of BBI. Such polymeric aggregates are capable of solubilization of dioleoyl BBI with a concomitant prevention of its inactivation at low pH values.

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Section: Methodsmentioning

confidence: 99%

Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

Kuskov¹,

Villemson²,

Штильман³

et al. 2007

J. Phys.: Condens. Matter

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Time behaviour of the modifier involved in the general mechanism of Botts and Morales assuming rapid equilibrium in the modifier bindings

et al. 2005

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Kinetic behavior of the general modifier mechanism of Botts and Morales with non-equilibrium binding

Chen

Liu

Qian

et al. 2012

Journal of Theoretical Biology

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In this paper, we perform a complete analysis of the kinetic behavior of the general modifier mechanism of Botts and Morales in both equilibrium steady states and non-equilibrium steady states (NESS). Enlightened by the non-equilibrium theory of Markov chains, we introduce the net flux into discussion and acquire an expression of product rate in NESS, which has clear biophysical significance. Up till now, it is a general belief that being an activator or an inhibitor is an intrinsic property of the modifier. However, we reveal that this traditional point of view is based on the equilibrium assumption. A modifier may no longer be an overall activator or inhibitor when the reaction system is not in equilibrium. Based on the regulation of enzyme activity by the modifier concentration, we classify the kinetic behavior of the modifier into three categories, which are named hyperbolic behavior, bellshaped behavior, and switching behavior, respectively. We show that the switching phenomenon, in which a modifier may convert between an activator and an inhibitor when the modifier concentration varies, occurs only in NESS. Effects of drugs on the Pgp ATPase activity, where drugs may convert from activators to inhibitors with the increase of the drug concentration, are taken as a typical example to demonstrate the occurrence of the switching phenomenon.

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Cited by 9 publications

References 19 publications

Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

Time behaviour of the modifier involved in the general mechanism of Botts and Morales assuming rapid equilibrium in the modifier bindings

Kinetic behavior of the general modifier mechanism of Botts and Morales with non-equilibrium binding

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