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Partitioning behavior of amino acids in aqueous two-phase systems containing polyethylene glycol and phosphate buffer

“…Zaslavsky [3] has published a monograph covering most topics in the area of aqueous two-phase partitioning. In the second half of the 20th century, there has been increasing attention focused on ATPSs in laboratory scale, especially for the separation of proteins, cells and other biological materials which require mild aqueous environments [4][5][6][7][8][9][10]. The most common ATPSs used for the separation of biomolecules are polyethylene glycol (PEG)/dextran or PEG/salt systems because of low-cost, rapid mass transfer, and phase equilibrium.…”

Purification of hyperthermophilic archaeal amylolytic enzyme (MJA1) using thermoseparating aqueous two-phase systems

“…Application of ATPS has also been extended to paper pulp processing for deligninfication [3][4][5]. Most recently, novel aqueous two-phase systems have been developed based on stimuli-responsive polymers, i.e., polymers capable of reversible phase transition in response to small changes in the environment, such as changes in pH or temperature [6][7][8]. The ethylene oxide-propylene oxide random copolymer (PEO-PPO), which is linear and non-ionic, has a decreased solubility in water at higher temperatures.…”

Continuous extraction of α- and β-amylases from Zea mays malt in a PEG4000/CaCl2 ATPS

“…It is possible to note that the protein is concentrated into the PEG-rich phase (top phase) and shows that PEG has good affinity for biomolecules in maize malt. Due to the high molecular weight or the high surface area of the protein and, the hydrophobic properties of PEG phase systems and of proteins are the dominant factors [1][2][3][10][11][12][13][14][15][16]21,28].…”