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Abstract

Three rice mapping populations of 208 BC 1 F 2 , 333 BC 3 F 2 and 335 F 2 lines derived from crosses of PTB33 × RD6, Rathu Heenati × KDML105 and IR71033-121-15 × KDML105, respectively, were used to detect brown planthopper (BPH) resistance genes. The modified mass tiller screening (MMTS) method was applied to evaluate the BPH resistance of all mapping population lines at the tillering stage. The BPH resistance genes detected from the BC 1 F 2 , BC 3 F 2 and F 2 populations were mapped in the same genomic region on the short arm of chromosome 6. The tightly linked markers RM589 and RM586 could explain 59.8%, 28.2% and 57.4 % of the phenotypic variance of the BPH resistance from the BC 1 F 2 , F 2 and BC 3 F 2 , respectively. The tightly linked SSR markers identified from this study should be useful in marker-assisted breeding to produce BPH resistant cultivars.

The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

“…no. IRGC 11730), that originated from Sri Lanka, carries BPH3 and BPH17 [16,39]. T65 was crossed with these donor parents and F 1 plants were backcrossed four times with T65 to generate BC 4 F 1 plants (Figure 3).…”

“…A number of highly resistant rice breeding lines and varieties contain several genes with resistance to BPH and other phloem feeding Hemiptera. These include IR71033-121-15 introgressed from Oryza minuta carrying BPH20 , BPH21 and qBPH6(t) [17,39]; Rathu Heenati carrying BPH3 , BPH17 , QBPH4.1 and QBPH4.2 [8,13,27]; and PTB33 carrying BPH2 and BPH32 [12,40,41].…”