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Woo, Byung Ho; Ge, Jingping; Jo, Young Tak; DeLuca, Patrick P.

doi:10.1023/a:1013090700443

Cited by 77 publications

(23 citation statements)

References 18 publications

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“…Measured values were then converted to particle sizes via the Stokes-Einstein relationship 36 . NIPAm particle size decreased with increasing temperature (Figure 2A), which is a distinctive characteristic of thermo responsive hydrogels [28][29][30][31][32][33] . The NIPAm/AAc particles showed a similar temperature dependence with respect to particle size, however they also demonstrated a pH dependent behavior ( Figure 2B).…”

mentioning

confidence: 97%

Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation

Luchini

Geho²,

Bishop³

et al. 2007

Nano Lett.

177

249

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Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: a) molecular size sieving b) affinity capture of all solution phase target molecules, and c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.There is an urgent need to discover novel biomarkers that provide sensitive and specific disease detection1 , 2. Cancer is rapidly becoming the leading cause of death for many population groups in the United States, largely due to the fact that the disease is usually diagnosed after the cancer has metastasized and treatment is ineffective. It is widely believed that early detection of cancer prior to metastasis will lead to a dramatic improvement in treatment outcome. Biomarkers are nucleic acids, proteins, protein fragments or metabolites indicative of a specific biological state, that are associated with the risk of contraction or presence of disease3. Biomarker research has revealed that low-abundance circulating proteins and peptides present a rich source of information regarding the state of the organism as a whole 4 . Two major hurdles have prevented these discoveries from reaching clinical benefit: 1) disease-relevant biomarkers in blood or body fluids may exist in exceedingly low concentrations within a complex mixture of biomolecules and could be masked by high-abundance species such as albumin, and 2) degradation of protein biomarkers can occur immediately following the collection of blood or body fluid as a result of endogenous or exogenous proteinases. The goal of this study was to create "smart" nano-particles that allow enrichment and encapsulation of selected classes of proteins and peptides from complex mixtures of biomolecules such as plasma, and protect them from degradation during subsequent sample handling. The captured analytes can be readily extracted from the particles by electrophoresis allowing for subsequent quantitative analysis. This nanotechnology provides a powerful tool that is uniquely suited for the discovery of novel biomarkers for early stage diseases such as cancer.SUPPORTING INFORMATION AVAILABLE: Available in the Supplementary Information are details on particles synthesis protocol, SDS PAGE analysis on molecular sieving properties and enzymatic degradation, and tables (Table S1 and S2) listing proteins (with peptide coverage lists) identified via LC-MS/MS (ESI) on material electroeluted from NIPAm and NIPAm/AAc particles. This material is available free of charge via the Internet at http://pubs.acs.org. NIH Public AccessAuthor Manuscript Nano Lett. Author manuscript; available in PMC 2010 May 28. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptThe concentration of proteins and peptides comprising t...

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mentioning

confidence: 97%

Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation

Luchini

Geho²,

Bishop³

et al. 2007

Nano Lett.

177

249

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“…68, 69, 70 Biomaterials ranging from natural polymers, such as alginate, fibrin or gelatin, to synthetic polymers, such as poly(lactic acid) and PLGA, have been fabricated with either single or multiple bioactive molecules. 71 These scaffolds have demonstrated an ability to stimulate and induce neighboring bone marrow stromal cells and enhance bone tissue formation. Many clinical applications have been conducted for bone regeneration in situ using calcium phosphate cements, collagen gel or sponge combined with clinically approved bone morphogenetic protein-2.…”

Section: Applications Of In Situ Tissue Regenerationmentioning

confidence: 99%

In situ tissue regeneration through host stem cell recruitment

et al. 2013

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The field of tissue engineering has made steady progress in translating various tissue applications. Although the classical tissue engineering strategy, which involves the use of culture-expanded cells and scaffolds to produce a tissue construct for implantation, has been validated, this approach involves extensive cell expansion steps, requiring a lot of time and laborious effort before implantation. To bypass this ex vivo process, a new approach has been introduced. In situ tissue regeneration utilizes the body's own regenerating capacity by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the site of injury. This approach relies on development of a target-specific biomaterial scaffolding system that can effectively control the host microenvironment and mobilize host stem/progenitor cells to target tissues. An appropriate microenvironment provided by implanted scaffolds would facilitate recruitment of host cells that can be guided to regenerating structural and functional tissues.

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“…Many excipients such as carbohydrates in the form of sugars [11,24], starch [25] and heparin [24,26] and water soluble polymers including poloxamers [24] and poly(vinyl alcohol) (PVA) [11,27,28] have been used in the formulations to increase the stability of proteins through various mechanisms. Carbohydrates could efficiently maintain the stability of proteins by forming a hydration layer around the protein which means that more water molecules are found on the surface of the protein than in the bulk [29].…”

Section: Introductionmentioning

confidence: 99%

Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

Erdemli

Keskin

Tezcaner

2015

Materials Science and Engineering: C

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Abstract: The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.

Cited by 77 publications

References 18 publications

Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation

Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation

In situ tissue regeneration through host stem cell recruitment

Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

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