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Regulatory Role of Na,K-ATPase in the Growth of Heart Tissue Explants in Organotypic Culture

“…They were cultured in Petri dishes with collagen sublayer. Culturing was performed in a CO 2 incubator at 36.5 o C for 3 days [4]. The nutrient medium included 40% Hanks solution, 40% Eagle medium, 5% chicken embryonic extract, and 15% fetal bovine serum containing 0.5 U/ml insulin, 0.6% glucose, 2 mM glutamine, and 100 U/ml gentamicin.…”

Role of Na/K-ATPase in Regulation of Neurite Growth in Sensory Neurons

“…Experiments were carried out on explants (n=300) of sensory ganglia of chick embryos cultured for 3 days on a collagen matrix in Petri dishes at 36.5 o C [6]. The culture medium contained 40% Hank's solution, 40% Eagle's medium, 5% chick embryonic extract, and 15% fetal calf serum supplemented with insulin (0.5 U/ml), glucose (0.6%), glutamine (2 mM), and gentamicin (100 U/ml).…”

“…Endogenous ouabain, which is considered by some authors as a hormone, modulates activity of Na/K-ATPase in a dose-dependent manner [4,11]. Tissue culture technique made it possible to study bioactive substances under conditions, which exclude the influence of humoral, neural, hormonal, and other factors acting in the whole organism [6,8,10]. The organotypic culture of spinal ganglia of 10-12-day-old chick embryo is a classical test-system for the study of neurite growth [5,6,10].…”

“…Tissue culture technique made it possible to study bioactive substances under conditions, which exclude the influence of humoral, neural, hormonal, and other factors acting in the whole organism [6,8,10]. The organotypic culture of spinal ganglia of 10-12-day-old chick embryo is a classical test-system for the study of neurite growth [5,6,10]. In neural tissue culture, the intensity of neurite growth clearly reflects stimulatory or inhibitory effects of examined agents.…”

Effect of amino acids and antibodies against nerve growth factor receptors on the development of organotypic culture of lymphoid tissue

“…Experiments on PC3 and DU145 cells of androgen-independent prostate cancer showed that methionine deprivation activated apoptosis in PC3 cells, while deprivation of tyrosine and phenylalanine primarily affected DU145 cells [13]. The stimulatory or inhibitory (increased apoptosis) effect of AA with charged side chain (lysine, arginine, asparagine, and glutamic acid) in organotypic culture of rat spleen depends on tissue maturity [3,4]. Organotypic culturing of tissues is one of the most reliable methods for screening of bioactive substances, because the criterion of bioactivity is changes in cell count in the explant growth zone due to cell proliferation or apoptosis [3,5,15].…”