“…These results are indicative that there is greater physical protection of AMF spores to predators, such as nematodes , in meso-and microaggregates than in macroaggregates; in contrast, the greatest amount of mycelium and production of glomalin favor the stabilization of these microaggregates (Wright & Upadhyaya, 1998;Rillig & Mummey, 2006;Vilela et al, 2014).…”
Section: Resultsmentioning
confidence: 96%
“…centrifugation at 1,720 g for 15 min. The supernatant protein was quantitated in mass spectrophotometer by the Bradford reagent assay, using bovine serum albumin as a standard (Wright & Upadhyaya, 1998). Total soil mycelium (TSM) was extracted by flotation in 45% sucrose, filtered on a gridded membrane, and stained with trypan blue, to enhance viewing in microscope, for quantification as described in Melloni & Cardoso (1999).…”
-The objective of this work was to evaluate the spore density and diversity of arbuscular mycorrhizal fungi (AMF) in soil aggregates from fields of "murundus" (large mounds of soil) in areas converted and not converted to agriculture. The experiment was conducted in a completely randomized design with five replicates, in a 5x3 factorial arrangement: five areas and three aggregate classes (macro-, meso-, and microaggregates). The evaluated variables were: spore density and diversity of AMF, total glomalin, total organic carbon (TOC), total extraradical mycelium (TEM), and geometric mean diameter (GMD) of soil aggregates. A total of 21 AMF species was identified. Spore density varied from 29 to 606 spores per 50 mL of soil and was higher in microaggregates and in the area with 6 years of conversion to agriculture. Total glomalin was higher between murundus in all studied aggregate classes. The area with 6 years showed lower concentration of TOC in macroaggregates (8.6 g kg -1) and in microaggregates (10.1 g kg -1). TEM was greater at the top of the murundus in all aggregate classes. GMD increased with the conversion time to agriculture. The density and diversity of arbuscular mycorrhizal spores change with the conversion of fields of murundus into agriculture.Index terms: diversity of arbuscular mycorrhizal fungi, geometric mean diameter, total glomalin, total mycelium, total organic carbon.
“…These results are indicative that there is greater physical protection of AMF spores to predators, such as nematodes , in meso-and microaggregates than in macroaggregates; in contrast, the greatest amount of mycelium and production of glomalin favor the stabilization of these microaggregates (Wright & Upadhyaya, 1998;Rillig & Mummey, 2006;Vilela et al, 2014).…”
Section: Resultsmentioning
confidence: 96%
“…centrifugation at 1,720 g for 15 min. The supernatant protein was quantitated in mass spectrophotometer by the Bradford reagent assay, using bovine serum albumin as a standard (Wright & Upadhyaya, 1998). Total soil mycelium (TSM) was extracted by flotation in 45% sucrose, filtered on a gridded membrane, and stained with trypan blue, to enhance viewing in microscope, for quantification as described in Melloni & Cardoso (1999).…”
-The objective of this work was to evaluate the spore density and diversity of arbuscular mycorrhizal fungi (AMF) in soil aggregates from fields of "murundus" (large mounds of soil) in areas converted and not converted to agriculture. The experiment was conducted in a completely randomized design with five replicates, in a 5x3 factorial arrangement: five areas and three aggregate classes (macro-, meso-, and microaggregates). The evaluated variables were: spore density and diversity of AMF, total glomalin, total organic carbon (TOC), total extraradical mycelium (TEM), and geometric mean diameter (GMD) of soil aggregates. A total of 21 AMF species was identified. Spore density varied from 29 to 606 spores per 50 mL of soil and was higher in microaggregates and in the area with 6 years of conversion to agriculture. Total glomalin was higher between murundus in all studied aggregate classes. The area with 6 years showed lower concentration of TOC in macroaggregates (8.6 g kg -1) and in microaggregates (10.1 g kg -1). TEM was greater at the top of the murundus in all aggregate classes. GMD increased with the conversion time to agriculture. The density and diversity of arbuscular mycorrhizal spores change with the conversion of fields of murundus into agriculture.Index terms: diversity of arbuscular mycorrhizal fungi, geometric mean diameter, total glomalin, total mycelium, total organic carbon.
“…The two GRSP fractions, total (T-GRSP) and easily extractable (EE-GRSP), were distinguished as a function of extraction conditions (Wright and Upadhyaya, 1998). Both fractions were extracted in auclave -EE-GRSP with 1 g of soil in 8.0 mL of 20 mmoL L -1 sodium citrate solution (pH 7.4) at 121 °C for 30 min, and T-GRSP with 1 g of soil in 8.0 mL of 50 mmoL L -1 sodium citrate solution (pH 8.0) at 121 °C for 60 min.…”
ABSTRACT:The removal of original vegetation for crops and pasture production and then followed by natural regeneration is a standard practice in the Atlantic Forest, which has produced patches with different degrees of degradation and regeneration across the landscape. The aim of this study was to evaluate the effects of replacement of native forest by pasture and natural regeneration of vegetation on soil and on soil organic matter (SOM) dynamics in the dry and rainy season in an Atlantic Forest fragment in Passa Vinte, Minas Gerais (MG), Brazil. Soil samples were collected in the rainy and dry season, at a depth of 0.00-0.05 m. The variables determined were total organic carbon (TOC) and particle-size fractions of SOM [particulate organic carbon
“…Levels of P, Ca and Mg in the soil were negatively correlated with the production of glomalin (Lovelock et al, 2004). High level of Fe in the AN and AI areas indicates the importance of this element for glomalin production, considering that up to 8% of Fe is present in its structure (Wright and Upadhyaya, 1998).…”
Section: Discussionmentioning
confidence: 92%
“…Fluorescein Diacetate hydrolytical Activity (FDA) was estimated according to Swisher and Carroll (1980) and the results expressed in µg fluorescein g −1 of dry soil h −1 . Two glomalin fractions-Easily Extractable (EEG) and Total (TG)-were quantified according to Wright and Upadhyaya (1998). To separate the aggregate fractions (1-2 mm), the soil was sieved (0.84 mm and 1.41 mm mesh sizes).…”
Problem statement: Mining activities involve the removal of the vegetal cover and the soil organic layer, causing a severe environmental impact. In Northeast Brazil, 40% of the world's crude gypsum is found in a semiarid area, making this region responsible for 95% of the gypsum demand in the national market. Although economically important, this activity is harmful to the environment. Studies of soil microbiological and biochemical attributes can help in the identification of the limitations of impacted ecosystems, providing data to define strategies for sustainability of such environments. Approach: To evaluate and compare the biological state of preserved and mining degraded semiarid soils, a native preserved area and areas impacted by gypsum mining were selected at the Araripina Experimental Station, located in the semiarid region of Pernambuco State, Northeast Brazil. The four sampling areas included: (1) A native, preserved "caatinga" area with spine bearing trees and shrubs and some characteristic xerophytic plants (AN); (2) An area surrounding the mine, presenting the same type of vegetation although already degraded (AM); (3) A waste deposit area (AR); (4) Interface area between the waste deposit and a mining degraded area (AI). Samples were taken in each area (1000 m 2 ) during two periods: wet (December/2003, Rainfall = 28.7 mm) and dry (September/2004, Rainfall = 1.3 mm). Results: Fluorescein diacetate hydrolysis values, microbial biomass C and basal respiration were higher in the preserved "caatinga" than in the impacted areas. The gypsum mining activity reduced the concentration of easily extractable glomalin in relation to the native "caatinga" area in both sampling periods. Higher deposits of total glomalin also occurred in the native area, however, mainly during the wet period. Conclusion: The mining activity produced a negative impact on the soil microbiota, reducing the total enzymatic activity. The microbial biomass was significantly lower in the waste deposit area than in the native and interface areas. The results indicated that the mining activity is harmful to the soil microbiota in this area and that glomalin can be a useful indicator of soil disturbance.
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