Untitled

M.D.L.A., Blanco; Nieves, Nadina; Sánchez, Margelys; Borroto, C.G; Castillo, R; González, José Ignacio López; Escalona, Maritza; Baeza, E.J.; Hernández, Zully

doi:10.1023/a:1005963925773

Cited by 26 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, efforts have been made to describe somatic embryogenesis at the molecular level using proteomic approaches in D. carota (Choi and Sung 1984;Dodeman and Ducreux 1996), M. sativa (Aleith and Richter 1990;Giroux and Pauls 1996;Giroux and Pauls 1997;Thibaud-Nissen et al 2003), sugarcane (Saccharum sp. ; Blanco et al 1997), Picea glauca (Stasolla et al 2004;Lippert et al 2005), and Vitis vinifera (Milena et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Bian

Zheng

et al. 2009

Plant Mol Biol Rep

View full text Add to dashboard Cite

Cyclamen persicum Mill. is a widely grown ornamental species that is clonally propagated by somatic embryogenesis. To better understand the biology of somatic embryo development in C. persicum, detailed proteomic (two-dimensional gel electrophoresis) and mass spectrometric analyses of somatic embryos at globular, torpedo, and germinating stages of development, along with nonembryogenic callus and zygotic embryos, were conducted. Of~460 proteins resolved in two-dimensional gels, 35 proteins were differentially expressed and could be reproducibly displayed across an isoelectric focusing range of 5 to 8. Among those proteins, five were constitutively expressed, 13 were upregulated, nine were downregulated, and eight were deemed as novel proteins during the torpedo stage. A total of 35 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and only four proteins were identified and these were available in public protein databases. The remaining protein spots were subsequently analyzed by MALDI-TOF-TOF-MS, and six proteins were then identified. These findings suggested that specific proteins are involved in the regulation of somatic embryogenesis.

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Bian

Zheng

et al. 2009

Plant Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…In other study, TAYLOR et al (1992) described the induction of organogenic and embryogenic callus in 18 cultivars, as well as to obtain cell cultures in suspension. Plant regeneration from embryogenic callus obtained from immature leaves was observed by DIBAX et al (2011), NIEVES et al (2008, VICKERS et al (2005) and BLANCO et al (1997) and using MS medium (MURASHIGE & SKOOG, 1962) supplemented with 2,4 dichlorophenoxyacetic acid (2,4-D) and LIU (1993), using different combinations of 2,4-D, kinetin (KIN), benzylaminopurine (BAP) and calcium pantothenate. For in vitro plant regeneration it is necessary to carry out histological analyses to determine the route of regeneration in order to discover whether the origins of the plant are in somatic embryogenesis or organogenesis, and to determine whether or not this is a reliable technique for use in the genetic transformation of sugarcane.…”

Section: Introductionmentioning

confidence: 97%

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

et al. 2012

View full text Add to dashboard Cite

The use of 2D‐DIGE to understand the regeneration of somatic embryos in avocado

et al. 2013

View full text Add to dashboard Cite

Avocado embryogenic cell cultures can be classified into two groups based on their morphology when cultured on a medium containing auxin: somatic embryo (SE) and proembryonic masses (PEM) type cultures. The calli of SE-type cell lines are able to go through the maturation process, whereas the calli of PEM cell lines rarely mature. We have investigated four independent avocado cell cultures (two SE and two PEM). The aim of this study was to link the differential regeneration capacity of the four cell cultures to a proteomic pattern and to gain insight into the regeneration capacity. A 2D-DIGE analysis followed by a blind multivariate analysis was able to separate the two SE lines from the PEM lines indicating that the protein profiles of SE and PEM calli are different. Based on the variable importance, that is, the differential protein pattern, we hypothesize that the regeneration capacity in avocado is correlated to the ability to overcome the physicochemical stress stimuli associated with the in vitro culture. Our identical culture conditions do not seem to trigger an appropriate response in PEM lines.

show abstract

Untitled

Cited by 26 publications

References 14 publications

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

The use of 2D‐DIGE to understand the regeneration of somatic embryos in avocado

Contact Info

Product

Resources

About