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Genetic Diversity of Pigeonpea (<i>Cajanus cajan</i> (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers

“…It was concluded that most of the diversity was among the wild relatives of pigeon pea or between the wild and the cultivated species, but not among the cultivated accessions. Very low level of genetic diversity in pigeonpea cultivras was reported also by many other researchers [9,10,13]. Such narrow genetic base can put a serious impediment to breeding progress in pigeon pea [23].…”

“…With the development of environmentally neutral, reliable and plant growth independent molecular markers, many researchers initiated the pigeonpea genetic diversity analysis. Assessment of genetic variability has been done using various molecular markers viz., RAPD [7][8][9], amplified fragment length polymorphism (AFLP) [3,10,11], restriction fragment length polymorphism (RFLP) [11,12], simple sequence repeat (SSR) [11,13], PCR-RFLP [14]. The present study was therefore, aimed to assess genetic diversity among pigeonpea cultivars and its related wild species using RAPD markers.…”

Organellar Genome Diversity in <i>Saccharum</i> and <i>Erianthus</i> spp. revealed by PCR-RFLP

“…The PCR-RFLP approach to study organellar genome diversity has been reported in many plants (Tsumura et al, 1995(Tsumura et al, , 1996Lakshmi et al, 2000;Parani et al, 2000Parani et al, , 2001Komatsu et al, 2001;Kishimoto et al, 2003;Nwakanma et al, 2003;Zhu et al, 2003;Asadi Abkenar et al, 2004Van Droogenbroeck et al, 2004;Ibrahim et al, 2007;Sehgal et al, 2008;Jena et al, 2009;Poczai et al, 2011). This work aimed at deciphering the polymorphism in the chloroplast and mitochondrial genomes in the Saccharum spp.…”

“…Specific chloroplast genes and/or intergenic spacers can be amplified (Taberlet et al, 1991;Demesure et al, 1995;Tsumura et al, 1995Tsumura et al, , 1996Dhingra and Folta, 2005;Heinze, 2007). The amplicons can be directly sequenced or restriction endonuclease digested (PCR-RFLP) or subjected to cleaved amplified polymorphic sequence (Tsumura et al, 1995(Tsumura et al, , 1996Lakshmi et al, 2000;Parani et al, 2000Parani et al, , 2001Komatsu et al, 2001;Kishimoto et al, 2003;Nwakanma et al, 2003;Zhu et al, 2003;Asadi Abkenar et al, 2004Van Droogenbroeck et al, 2004;Ibrahim et al, 2007;Sehgal et al, 2008;Jena et al, 2009;Poczai et al, 2011).…”

Comparative Analysis of Genetic Diversity among Cultivated Pigeonpea (&lt;i&gt;Cajanus cajan&lt;/i&gt; (L) Millsp.) and Its Wild Relatives (&lt;i&gt;C. albicans&lt;/i&gt; and &lt;i&gt;C. lineatus&lt;/i&gt;) Using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Fingerprinting

“…DNA markers provide an opportunity to characterize genotypes and to measure genetic relationships more precisely than other markers [5]. Assessment of genetic variability in crops has been done using various molecular markers viz., RAPD [6]- [8], AFLP [9]- [11], RFLP [11] [12], SSR [11] [13], PCR-RFLP [14]. RAPDs are generated by PCR amplification using single, short, synthetic, random oligonucleotide as a primer that acts as both forward as well as reverse primer.…”