Two RAPD markers linked to gene for resistance (assayed as pustule number cm −2 leaf area) to rust [Uromyces fabae (Pers.) de Bary] in pea (Pisum sativum L.) were identified using a mapping population of 31 BC 1 F 1 [HUVP 1 (HUVP 1 × FC 1] plants, FC 1 being the resistant parent. The analysis of genetics of rust resistance was based on the parents, generations. Rust resistance in pea is of non-hypersensitive type; it appeared to be governed by a single partially dominant gene for which symbol Ruf is proposed. Further, this trait seems to be affected by some polygenes in addition to the proposed oligogene Ruf. A total of 614 decamer primers were used to survey the parental polymorphism with regard to DNA amplification by polymerase chain reaction. The primers that amplified polymorphic bands present in the resistant parent (FC 1) were used for bulked segregant analysis. Those markers that amplified consistently and differentially in the resistant and susceptible bulks were separately tested with the 31 BC 1 F 1 individuals. Two RAPD makers, viz., SC10-82 360 (primer, GCCGTGAAGT), and SCRI-71 1000 (primer, GTGGCGTAGT), flanking the rust resistance gene (Ruf) with a distance of 10.8 cM (0.097 rF and LOD of 5.05) and 24.5 cM (0.194 rF and a LOD of 2.72), respectively, were identified. These RAPD markers were not close enough to Ruf to allow a dependable maker-assisted selection for rust resistance. However, if the two makers flanking Ruf were used together, the effectiveness of MAS would be improved considerably.
The global malnutrition burden imparts long-term developmental, economic, social, and medical consequences to individuals, communities, and countries. The current developments in biotechnology have infused biofortification in several food crops to fight malnutrition. However, these methods are not sustainable and suffer from several limitations, which are being solved by the CRISPR-Cas-based system of genome editing. The pin-pointed approach of CRISPR-based genome editing has made it a top-notch method due to targeted gene editing, thus making it free from ethical issues faced by transgenic crops. The CRISPR-Cas genome-editing tool has been extensively used in crop improvement programs due to its more straightforward design, low methodology cost, high efficiency, good reproducibility, and quick cycle. The system is now being utilized in the biofortification of cereal crops such as rice, wheat, barley, and maize, including vegetable crops such as potato and tomato. The CRISPR-Cas-based crop genome editing has been utilized in imparting/producing qualitative enhancement in aroma, shelf life, sweetness, and quantitative improvement in starch, protein, gamma-aminobutyric acid (GABA), oleic acid, anthocyanin, phytic acid, gluten, and steroidal glycoalkaloid contents. Some varieties have even been modified to become disease and stress-resistant. Thus, the present review critically discusses CRISPR-Cas genome editing-based biofortification of crops for imparting nutraceutical properties.
Genetic diversity among and between 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its wild relatives (C. albicans and C. lineatus) analysed using RAPD. Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 -0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. C. albicans and C. lineatus showed only 0.231 similarity with each other and C. albicans showed relatively higher similarity with C. cajan cultivars than that showed by C. lineatus. In dendrogram the 16 cultivars grouped into two distinct clusters comprising of seven and nine genotypes each while the wild species form out groups. Bootstrap analysis of the dendrogram was performed and resulted in significant bootstrap values. Principal components analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 55.9%, 5.9%, and 5.6% of the variation, respectively, with cumulative variation of the first three PCs was 67.4%.
In an alarming tale of agricultural excess, the relentless overuse of chemical fertilizers in modern farming methods have wreaked havoc on the once-fertile soil, mercilessly depleting its vital nutrients while inflicting irreparable harm on the delicate balance of the surrounding ecosystem. The excessive use of such fertilizers leaves residue on agricultural products, pollutes the environment, upsets agrarian ecosystems, and lowers soil quality. Furthermore, a significant proportion of the nutrient content, including nitrogen, phosphorus, and potassium, is lost from the soil (50–70%) before being utilized. Nanofertilizers, on the other hand, use nanoparticles to control the release of nutrients, making them more efficient and cost-effective than traditional fertilizers. Nanofertilizers comprise one or more plant nutrients within nanoparticles where at least 50% of the particles are smaller than 100 nanometers. Carbon nanotubes, graphene, and quantum dots are some examples of the types of nanomaterials used in the production of nanofertilizers. Nanofertilizers are a new generation of fertilizers that utilize advanced nanotechnology to provide an efficient and sustainable method of fertilizing crops. They are designed to deliver plant nutrients in a controlled manner, ensuring that the nutrients are gradually released over an extended period, thus providing a steady supply of essential elements to the plants. The controlled-release system is more efficient than traditional fertilizers, as it reduces the need for frequent application and the amount of fertilizer. These nanomaterials have a high surface area-to-volume ratio, making them ideal for holding and releasing nutrients. Naturally occurring nanoparticles are found in various sources, including volcanic ash, ocean, and biological matter such as viruses and dust. However, regarding large-scale production, relying solely on naturally occurring nanoparticles may not be sufficient or practical. In agriculture, nanotechnology has been primarily used to increase crop production while minimizing losses and activating plant defense mechanisms against pests, insects, and other environmental challenges. Furthermore, nanofertilizers can reduce runoff and nutrient leaching into the environment, improving environmental sustainability. They can also improve fertilizer use efficiency, leading to higher crop yields and reducing the overall cost of fertilizer application. Nanofertilizers are especially beneficial in areas where traditional fertilizers are inefficient or ineffective. Nanofertilizers can provide a more efficient and cost-effective way to fertilize crops while reducing the environmental impact of fertilizer application. They are the product of promising new technology that can help to meet the increasing demand for food and improve agricultural sustainability. Currently, nanofertilizers face limitations, including higher costs of production and potential environmental and safety concerns due to the use of nanomaterials, while further research is needed to fully understand their long-term effects on soil health, crop growth, and the environment.
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