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Liao, Zhihua; Chen, Min; Tan, Feng; Sun, Xiaoping; Tang, Kexuan

doi:10.1023/a:1025868515705

Cited by 46 publications

(9 citation statements)

References 7 publications

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“…Aloe species has been cultured in vitro by various workers [2][3][4][5][6][7][8][9]. Efficient plantlet regeneration for a selected genotype of A. vera L. sweet aloe was achieved through direct shoot bud proliferation using axillary shoot buds [10] and indirect shoot bud regeneration via an intervening callus phase using inflorescence axis as explants [11].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers

Rathore

Chikara

Mastan

et al. 2011

Appl Biochem Biotechnol

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Efficient plantlet regeneration with and without intermediate callus phase was achieved for a selected genotype of Aloe vera L. which is sweet in test and used as a vegetable and source of food. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) marker assays were employed to evaluate genetic stability of plantlets and validate the most reliable method for true-to-type propagation of sweet aloe, among two regeneration systems developed so far. Despite phenotypic similarities in plantlets produced through both regeneration systems, the differences in genomic constituents of plantlets produced through intermediate callus phase using soft base of inflorescence have been effectively distinguished by RAPD and ISSR markers. No polymorphism was observed in regenerants produced following direct regeneration of axillary buds, whereas 80% and 73.3% of polymorphism were observed in RAPD and ISSR, respectively, in the regenerants produced indirectly from base of the inflorescence axis via an intermediate callus phase. Overall, 86.6% of variations were observed in the plantlets produced via an intermediate callus phase. The occurrence of genetic polymorphism is associated with choice of explants and method used for plantlet regeneration. This confirms that clonal propagation of sweet aloe using axillary shoot buds can be used for commercial exploitation of the selected genotype where a high degree of fidelity is an essential prerequisite. On the other hand, a high degree of variations were observed in plantlets obtained through indirect regeneration and thus cannot be used for the mass multiplication of the genotype; however, it can be used for crop improvement through induction of somaclonal variations and genetic manipulations.

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Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers

Rathore

Chikara

Mastan

et al. 2011

Appl Biochem Biotechnol

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“…Abrie and van Staden [7] used seedling-derived explants for micropropagation of Aloe polyphylla, which is an endangered aloe species. Liao et al [8] developed a micropropagation technique of A. vera var. chinensis, which is another endangered taxa.…”

Section: Introductionmentioning

confidence: 99%

Plantlet Regeneration from Callus Cultures of Selected Genotype of Aloe vera L.—An Ancient Plant for Modern Herbal Industries

Rathore

Chikara

Shekhawat

2010

Appl Biochem Biotechnol

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Aloe vera L., a member of Liliaceae, is a medicinal plant and has a number of curative properties. We describe here the development of tissue culture method for high-frequency plantlet regeneration from inflorescence axis-derived callus cultures of sweet aloe genotype. Competent callus cultures were established on 0.8% agar-gelled Murashige and Skoog's (MS) basal medium supplemented with 6.0 mg l⁻¹ of 2,4-dichlorophenoxyacetic acid (2,4-D) and 100.0 mg l⁻¹ of activated charcoal and additives (100 mg l⁻¹ of ascorbic acid, 50.0 mg l⁻¹ each of citric acid and polyvinylpyrrolidone, and 25.0 mg l⁻¹ each of L-arginine and adenine sulfate). The callus cultures were cultured on MS medium containing 1.5 mg l⁻¹ of 2,4-D, 0.25 mg l⁻¹ of Kinetin (Kin), and additives with 4% carbohydrate source for multiplication and long-term maintenance of regenerative callus cultures. Callus cultures organized, differentiated, and produced globular embryogenic structures on MS medium with 1.0 mg l⁻¹ of 2,4-D, 0.25 mg l⁻¹ of Kin, and additives (50.0 mg l⁻¹ of ascorbic acid and 25.0 mg l⁻¹ each of citric acid, L-arginine, and adenine sulfate). These globular structures subsequently produced shoot buds and then complete plantlets on MS medium containing 1.0 mg l⁻¹ of 6-benzylaminopurine and additives. A hundred percent regenerated plantlets were hardened in the greenhouse and stored under an agro-net house/nursery. The regeneration system defined could be a useful tool not only for mass-scale propagation of selected genotype of A. vera, but also for genetic improvement of plant species through genetic transformation.

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“…The plant material can be disinfected with mercuric chloride (Liao et al, 2004; Ahmed et al, 2007; Kumar et al, 2011) which is now seldom used as it is dangerous (Kaviani, 2015), and sodium hypochlorite (Natali et al, 1990; Nayanakantha et al, 2010b; Adelberg and Naylor-Adelberg, 2012). Browning decreases the explant survival and leads to failures in shoot regeneration (Roy and Sarkar, 1991).…”

Section: Propagation Techniquesmentioning

confidence: 99%

“…A suitable carbon source and antioxidants (ascorbic and citric acids) need to be added to avoid excessive phenolic secretion in the culture medium (Das and Srivastav, 2015). To eliminate blacking and browning, PVP can be used (Roy and Sarkar, 1991; Liao et al, 2004; Rathore et al, 2011b). Nayanakantha et al (2010b) reported that a medium containing 1.0 g L −1 PVP + 10 mg L −1 citric acid + 500 mg L −1 AC resulted in blocking the browning of the medium due to tissue phenols release.…”

Section: Propagation Techniquesmentioning

confidence: 99%

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

2016

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Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

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Cited by 46 publications

References 7 publications

Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers

Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers

Plantlet Regeneration from Callus Cultures of Selected Genotype of Aloe vera L.—An Ancient Plant for Modern Herbal Industries

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

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