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Masci, Stefania; D’Ovidio, R.; Scossa, Federico; Patacchini, C.; Lafiandra, D.; Anderson, Olin D.; Blechl, Ann E.

doi:10.1023/a:1026346823301

Cited by 32 publications

(2 citation statements)

References 23 publications

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“…In addition to practical value, these works allow understanding the role of individual genes of high and low molecular weight glutenins in flour quality. Thus, a decline in the SDS-sedimentation index and flour strength was observed in transgenic bread wheat lines with enhanced expression of low molecular weight glutelin genes [24]. Simultaneous expression of three genes of HMW-GS, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…in the genomes of different lines and cultivars of bread wheat. The studies resulted in obtaining transgenic lines with improved quality of flour, increased dough rising potency and elasticity [20][21][22][23][24]. In addition to practical value, these works allow understanding the role of individual genes of high and low molecular weight glutenins in flour quality.…”

Section: Introductionmentioning

confidence: 99%

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GENETIC ENGINEERING AS A TOOL FOR MODIFICATION OF SEED STORAGE PROTEINS AND IMPROVEMENT OF NUTRITIONAL VALUE OF CEREAL GRAIN (review)

Elkonin¹,

Domanina²,

Ital’yanskaya³

2016

S-h. biol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GENETIC ENGINEERING AS A TOOL FOR MODIFICATION OF SEED STORAGE PROTEINS AND IMPROVEMENT OF NUTRITIONAL VALUE OF CEREAL GRAIN (review)

Elkonin¹,

Domanina²,

Ital’yanskaya³

2016

S-h. biol.

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Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line overexpressing a low molecular weight glutenin subunit gene in the endosperm

Scossa

Laudencia‐Chingcuanco

Anderson

et al. 2008

Proteomics

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We carried out a parallel transcriptional and proteomic comparison of seeds from a transformed bread wheat line that overexpresses a transgenic low molecular weight glutenin subunit gene relative to the corresponding nontransformed genotype. Proteomic analyses showed that, during seed development, several classes of endosperm proteins were differentially accumulated in the transformed endosperm. As a result of the strong increase in the amount of the transgenic protein, the endogenous glutenin subunit, all subclasses of gliadins, and metabolic as well as chloroform/methanol soluble proteins were diminished in the transgenic genotype. The differential accumulation detected by proteomic analyses, both in mature and developing seeds, was paralleled by the corresponding changes in transcript levels detected by microarray experiments. Our results suggest that the most evident effect of the strong overexpression of the transgenic glutenin gene consists in a global compensatory response involving a significant decrease in the amounts of polypeptides belonging to the prolamin superfamily. It is likely that such compensation is a consequence of the diversion of amino acid reserves and translation machinery to the synthesis of the transgenic glutenin subunit.

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Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat

Tamás-Nyitrai

Jones

Tamás

2012

Plant Cell Culture Protocols

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After rice, wheat is considered to be the most important world food crop, and the demand for high-quality wheat flour is increasing. Although there are no GM varieties currently grown, wheat is an important target for biotechnology, and we anticipate that GM wheat will be commercially available in 10-15 years. In this chapter, we summarize the main features and challenges of wheat transformation and then describe detailed protocols for the production of transgenic wheat plants both by biolistic and Agrobacterium-mediated DNA-delivery. Although these methods are used mainly for bread wheat (Triticum aestivum L.), they can also be successfully applied, with slight modifications, to tetraploid durum wheat (T. turgidum L. var. durum). The appropriate size and developmental stage of explants (immature embryo-derived scutella), the conditions to produce embryogenic callus tissues, and the methods to regenerate transgenic plants under increasing selection pressure are provided in the protocol. To illustrate the application of herbicide selection system, we have chosen to describe the use of the plasmid pAHC25 for biolistic transformation, while for Agrobacterium-mediated transformation the binary vector pAL156 (incorporating both the bar gene and the uidA gene) has been chosen. Beside the step-by-step methodology for obtaining stably transformed and normal fertile plants, procedures for screening and testing transgenic wheat plants are also discussed.

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Cited by 32 publications

References 23 publications

GENETIC ENGINEERING AS A TOOL FOR MODIFICATION OF SEED STORAGE PROTEINS AND IMPROVEMENT OF NUTRITIONAL VALUE OF CEREAL GRAIN (review)

GENETIC ENGINEERING AS A TOOL FOR MODIFICATION OF SEED STORAGE PROTEINS AND IMPROVEMENT OF NUTRITIONAL VALUE OF CEREAL GRAIN (review)

Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line overexpressing a low molecular weight glutenin subunit gene in the endosperm

Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat

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