Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line overexpressing a low molecular weight glutenin subunit gene in the endosperm

Abstract

We carried out a parallel transcriptional and proteomic comparison of seeds from a transformed bread wheat line that overexpresses a transgenic low molecular weight glutenin subunit gene relative to the corresponding nontransformed genotype. Proteomic analyses showed that, during seed development, several classes of endosperm proteins were differentially accumulated in the transformed endosperm. As a result of the strong increase in the amount of the transgenic protein, the endogenous glutenin subunit, all subclasses of gliadins, and metabolic as well as chloroform/methanol soluble proteins were diminished in the transgenic genotype. The differential accumulation detected by proteomic analyses, both in mature and developing seeds, was paralleled by the corresponding changes in transcript levels detected by microarray experiments. Our results suggest that the most evident effect of the strong overexpression of the transgenic glutenin gene consists in a global compensatory response involving a significant decrease in the amounts of polypeptides belonging to the prolamin superfamily. It is likely that such compensation is a consequence of the diversion of amino acid reserves and translation machinery to the synthesis of the transgenic glutenin subunit.

Applied Cell Biology of Sulphur and Selenium in Plants

“…In one case, rice was transformed to express an S-rich 2S sunflower storage protein at high levels, but the amino acid composition and the total protein content remained largely the same, due to concomitant downregulation of other, endogenous, storage proteins (Hagan et al 2003). In a different case, the overexpression of seed glutenin caused a decrease in the contents of endogenous prolamins in wheat (Scossa et al 2008). These experiments suggest that a seed filling sensor may exist that monitors seed metabolite and/or volume and adjusts seed reserve contents (Lin et al 2013).…”

A new holistic exploratory approach to Systems Biology by Near Infrared Spectroscopy evaluated by chemometrics and data inspection

“…This is a consequence of self-organisation as forecasted by Prigogine [57] discussed in sections 5.2 and 5.3.5. Thus Scossa et al [61] over expressed a LMW glutenin subunit indicative for baking quality and obtained 250 uni-genes that where significantly expressed in the transcriptome. An explorative unsupervised strategy by NIR spectroscopy by PCA for orientation and by direct inspection of MSC-treated spectral intervals before data reduction by destructive mathematical models, is the hereto the only way to make an overview to 'escape reducing the complexity' that Wolkenhauer [24] did not consider as possible.…”

MS‐based analytical methodologies to characterize genetically modified crops

“…When prolamin composition of both manipulated lines was compared to their respective control lines, significant differences were found only in the GM Ofanto wheat cultivars (Di Luccia et al, 2005). In a later study, it was observed that several classes of proteins were differentially accumulated in the subproteome endosperm of wheat as a result of the overexpression of a low molecular weight glutenin subunit (LMW-GS) in a GM bread wheat that had modified visco-elastic properties of the derived dough (Scossa et al, 2008). The overexpression of LMW-GS was compensated by a decrease in the amount of polypeptides that belong to the prolamin superfamily.…”