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doi:10.1111/aab.2007.150.issue-2

Cited by 6 publications

(1 citation statement)

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“…[9][10][11] In olive (Olea europaea L.), two PIP (Plasma membrane Intrinsic Proteins) genes (OePIP1,1 OePIP2,1) and one TIP (Tonoplast Intrinsic Proteins) gene OeTIP1,1 showed a significant decrease at the transcript levels in leaves, roots, and twigs. 12,13 In the root of tobacco (Nicotiana tabacum L.), drought stress significantly reduced PIP transcript levels of two investigated genes, NtPIP1;1 and NtPIP2;1. 11,14,15 Aquaporins (AQPs) belong to the great family of conserved proteins termed MIPs (Major Intrinsic Proteins), and they exhibit a high diversity in numerous organisms.…”

Section: Introductionmentioning

confidence: 99%

In Silico development of new SSRs primer for aquaporin linked to drought tolerance in plants

Karim

Gaboun²,

Belkadi

et al. 2018

Plant Signaling & Behavior

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Plants are exposed to various stress factors including biotic and abiotic stresses. Drought is a limiting factor that minimizes the development and growth of several plants in arid and semi-arid regions. Stress response is usually occur at different levels, Morphological, physiological and biochemical while at the molecular level a large number of genes are involved. This study aims at developing a new SSR primer for aquaporin related to drought stress in plants. A total of 177 complete coding sequences (CDS) available in the NCBI database are downloaded. After analyzing with BLAST, 163 sequences are selected. 1294 SSR derived from these sequences are characterized with MISA and indicating that all sequences contained SSRs. The most abundant SSR has been tetra-nucleotide repeat motif (36%) and among all the tetra-nucleotide repeats, the motif AAAG/CTTT was the most common type, whereas in tri-nucleotide, the motif CCG/CGG has been the predominate type. By using Primer3, 1120 primer pairs are generated and after analyzing, only 735 non redundant primer pairs that present the good characteristics are selected. Among them, some of the pairs of primers are randomly selected and validated on DNA of various species using PCR and agarose gel. ARTICLE HISTORY

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