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Iembo, T.; Silva, Roberto da; Pagnocca, Fernando Carlos; Gomes, Eleni

doi:10.1023/a:1020774527212

Cited by 24 publications

(7 citation statements)

References 13 publications

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“…The p-glucosidase production obtained in the present work is more than average results found in the literature. Iembo et al (10) related a maximum production of 0.5 U/mL by Aureobasidium sp. in 168 h of culture in which wheat bran was used as substrate in SSF.…”

Section: Enzyme Productionmentioning

confidence: 99%

“…When compared with other microbial~-glucosidases found in literature (4,(22)(23)(24), this enzyme presented a very high level of thermal stability. Previous works (10,15,25) described the production of~-glucosidase from Aureobasidium Spa and A. pullulans as having noticeable stable levels of both pH and temperature. Other research conducted with stable enzymes disclosed very little difference between stable and unstable enzymes regarding amino acid's sequence.…”

Section: Enzyme Propertiesmentioning

confidence: 99%

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Production of Cellulolytic and Hemicellulolytic Enzymes From Aureobasidium pulluans on Solid State Fermentation

Leite¹,

Bocchini²,

Martins³

et al. 2007

Applied Biochemistry and Biotecnology

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This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL~-glucosidase,and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and~-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75°C, respectively. These enzymes remained stable between a wide range of pH. The~-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75°C for 1 h.

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Section: Enzyme Productionmentioning

confidence: 99%

Section: Enzyme Propertiesmentioning

confidence: 99%

Production of Cellulolytic and Hemicellulolytic Enzymes From Aureobasidium pulluans on Solid State Fermentation

Leite¹,

Bocchini²,

Martins³

et al. 2007

Applied Biochemistry and Biotecnology

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show abstract

“…Wheat bran is a complex substrate rich in proteins (14%), carbohydrates (27%), minerals (5%), fat (6%), and B-vitamin (17), this probably favored the growth and the production of enzymes for the microorganism. Previous works (3,(10)(11)(12)18) report the production of cellulases and hemicelulases, using derived wheat (bran and straw) as substrate. The highest endoglucanase and xylanase production was obtained after 96 h of fermentation in wheat bran-1.05 and 5.0 U/mL, respectively.…”

Section: Enzyme Productionmentioning

confidence: 99%

“…When compared with other microbial β-glucosidases found in literature (4,(22)(23)(24), this enzyme presented a very high level of thermal stability. Previous works (10,15,25) described the production of β-glucosidase from Aureobasidium sp. and A. pullulans as having noticeable stable levels of both pH and temperature.…”

Section: Enzyme Propertiesmentioning

confidence: 99%

“…Previous works described the production cellulases and hemicellulases using SSF and agricultural residues (2, [10][11][12]. Brazil is an agroindustrial country known for its production of soy, corn, sugar cane, cassava, coffee, and so on, and for its high consumption of wheat, which generate large amounts of residues that have considerable potential for SSF applications (13).…”

Section: Introductionmentioning

confidence: 99%

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Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation

Leite

Bocchini

Martins

et al. 2007

Appl Biochem Biotechnol

View full text Add to dashboard Cite

This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL beta-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and beta-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75 degrees C, respectively. These enzymes remained stable between a wide range of pH. The beta-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75 degrees C for 1 h.

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