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Eb, Moroshkina; Ne, Zagoruĭko; Dv, Ovchinnikov; Plekhanova, N. G.; En, Glibin

doi:10.1023/a:1019825923903

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…The absorption and CD spectra do not change over time. Similar curves have previously been obtained for compound II that does not form aggregates in aqueous salt solutions and interacts with DNA as dimers or monomers depending on the ratio of ligand and DNA molar concentrations (r = C I /C DNA ) [23]. Such a spectral behavior of compound I in the presence of DNA gives evidence of the absence of a free ligand in the solution in the whole studied range r (0.05 < r < 1.0), and consequently, of a high affinity of compound I to DNA in KCl solutions.…”

Section: Resultssupporting

confidence: 82%

Formation of the aggregates of actinocin derivatives containing 4′-benzo-15-crown-5 radicals in amide groups and their interaction with a DNA molecule

Sedova

Urusova

2011

J Struct Chem

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Methods of spectrophotometry, spectropolarimetry, and viscometry are used to study the self-organization in the solution of crown-containing actinocin derivative (I) exhibiting antitumor activity and the interaction of the formed aggregates with a DNA molecule. The presence of the 4′-benzo-15-crown-5 radical in the structure of the studied compound determines the observed differences in its complexation with Na + and K + ions. The process of aggregation in the presence of K + ions is accompanied by a shift of the long-wave band in the absorption spectrum to short-wave (the formation of H type aggregates) or long-wave (the formation of J type aggregates) regions depending on the K + ion concentration in the solution. In the presence of Na + ions, regardless of their concentration in the solution, J type aggregates form. A scheme of complex formation and their mutual transformations with changes in the ionic composition of the medium is proposed. A study of the interaction of this compound with DNA shows that in the presence of K + ions it binds to the DNA molecule in the form of monomers and/or dimers without producing large supramolecular aggregates. The H and J structures formed in K + -containing solutions of compound I are broken in the interaction with DNA. If a solution of compound I is added to a DNA solution containing Na + ions, the J type aggregates are formed directly on the surface of the DNA molecule. At the same type, the J structures originally formed in the Na + -containing solution of compound I practically do not interact with DNA. A study of this system shows that the introduction of the crown group in the compound molecule with a heterocyclic chromophore provides the opportunity to affect its affinity and binding to the DNA molecule by means of the ionic composition of the medium.

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Section: Resultssupporting

confidence: 82%

Formation of the aggregates of actinocin derivatives containing 4′-benzo-15-crown-5 radicals in amide groups and their interaction with a DNA molecule

Sedova

Urusova

2011

J Struct Chem

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“…In this case the chromophore intercalates into double helix, or it locates in the minor groove of the DNA double helix. Previously, it was shown on the interaction of DNA with derivatives of actinocine [4], xanthone [3], phenazine [5] that the localization of molecule of the ligand upon binding is determined by the ionic conditions [3] and its structural features [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The interaction of DNA molecule with new synthetic compounds, piperazine derivatives of benzo [4,5]imidazo[1,2-a]phthalazine, was investigated by the spectral, hydrodynamic and optical methods. The thermodynamic parameters of the interaction and the stoichiometry of the complexes were determined by spectrophotometric titration.…”

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confidence: 99%

The interaction of DNA with piperazine derivatives of benzoimidazophthalazine

Osinnikova

Travkina

2016

J. Phys.: Conf. Ser.

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Abstract. The interaction of DNA molecule with new synthetic compounds, piperazine derivatives of benzo [4,5]imidazo[1,2-a]phthalazine, was investigated by the spectral, hydrodynamic and optical methods. The thermodynamic parameters of the interaction and the stoichiometry of the complexes were determined by spectrophotometric titration. A mode of binding and structure of the complexes were determined by analyzing the changes in the intrinsic viscosity and the optical anisotropy of the macromolecule upon complexation. It is shown that an increase in the intrinsic viscosity and the optical anisotropy with a small content of the ligand in the complex is caused by the increase in the thermodynamic rigidity of macromolecules upon the formation of the complex. Increase of the contour length of the macromolecule in the complex does not occur, that indicates nonintercalative mode of binding the piperazine derivatives of benzoimidazophthalazine with DNA.

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“…-in the case of compound III (Scheme 1) and relat ed structures [23,24] (which share properties of "classic bis intercalators"), intercalation of both "functional" (ethidium and acridine) fragments into polynucleotide can take place only if the length of the linker joining these fragments together exceeds some critical value; otherwise, intercalation of only one (ethidium) fragment into nucleic acid would be observed and the second (acri dine) fragment binds to the external part of the polynu cleotide; -at r < 0.07 (r is the amount of bound ligand per unit of substrate concentration) compound IV (Scheme 1) interacts by intercalation due to the presence of its bi thiazole fragments, whereas at r > 0.07 it binds non specifically to polynucleotide due to the presence of the phenoxazine moiety, which itself can exhibit intercalating properties under other conditions [25]; -at n = 1 (i.e., when one CH 2 group links the phe noxazine moiety with the benzcrown fragment of the lig and molecule) and r < 0.1 compounds V (Scheme 1) interact with nucleic acids by intercalation due to the phenoxazine moiety; at n = 0 or 2, or at n = 1 with r > 0.1 they bind to nucleic acids via their benzcrown fragments, whereas at n > 2 they poorly interact with nucleic acids [26];…”

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confidence: 99%

Study of Applicability of the Bifunctional System “Ethidium Bromide + Hoechst-33258” for DNA Analysis

Sibirtsev

2005

Biochemistry (Moscow)

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Changes in absorbance and fluorescence excitation and emission spectra in the ultraviolet and visible regions of the system containing ethidium bromide (EtBr) and Hoechst-33258 (Ht) were investigated depending on various DNA quantities and the composition of the medium. It is noted that spectral properties of this system are determined by interactions of EtBr and Ht both with nucleic acid and with one another (for example, joint sorption of EtBr and Ht on DNA may involve fluorescent resonance energy transfer between the dye molecules). Thus, different modes of EtBr and Ht specificity to substrate and assay conditions suggest that combined use of these dyes provides some additional effects that may be interesting in terms of structure-functional study of nucleic acid. Some of these effects are considered in this paper.

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Cited by 4 publications

References 5 publications

Formation of the aggregates of actinocin derivatives containing 4′-benzo-15-crown-5 radicals in amide groups and their interaction with a DNA molecule

Formation of the aggregates of actinocin derivatives containing 4′-benzo-15-crown-5 radicals in amide groups and their interaction with a DNA molecule

The interaction of DNA with piperazine derivatives of benzoimidazophthalazine

Study of Applicability of the Bifunctional System “Ethidium Bromide + Hoechst-33258” for DNA Analysis

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