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Abstract

We have previously identified two cDNAs encoding vegetative storage proteins (VSPs) in Arabidopsis thaliana. Unlike soybean in which VSPs accumulate at high levels in leaves, A. thaliana VSP mRNAs are abundant in flowers. To understand tissue-specific expression and possible roles of VSPs on reproductive organ development, genes corresponding to VSPs (Vsp1 and Vsp2) and their putative promoters were characterized in this study. Genomic sequence analysis revealed that Vsp1 and Vsp2 resemble each other except in their introns, and that these two genes were organized in a tandem array with an interval of 6 kb in a region. The expression patterns of Vsp1 and Vsp2 were examined using transgenic A. thaliana plants carrying a promoter from Vsp1 or Vsp2 fused to a bacterial beta-glucuronidase (GUS) reporter gene. The promoter from Vsp1 expressed its effect in gynoecia, especially in styles, the basal and distal ends of ovaries and in siliques, whereas the promoter from Vsp2 showed its activity in vegetative shoots, petioles, peduncles and receptacles of floral organs. These results suggest that expression of Vsp1 and Vsp2 may be developmentally regulated in A. thaliana. In the transgenic plants, the GUS activity was induced by wounding in an area around the mid-rib of leaves. Therefore, Vsp1 and Vsp2 promoters appear to have elements required for both tissue specificity and wounding.

Potassium deficiency induces the biosynthesis of oxylipins and glucosinolates in Arabidopsis thaliana

“…Previous microarray experiments indicated reversible induction by low K of genes closely related to JA [6], particularly VSP2 (At5g24770, [29]), a well known target of JA-signaling, and LOX2 (At3g45140) encoding a 13(S)-lipoxygenase (LOX), which catalyses the initial step of JA production [30]. We monitored the time course of this induction over progressing K-deficiency and found that both genes are already induced at day 12 (before visible symptoms appear) and experience a further sharp rise in transcription over the following days (Fig.…”

Different Transcript Patterns in Response to Specialist and Generalist Herbivores in the Wild Arabidopsis Relative Boechera divaricarpa

“…Two notable exceptions were At5g24770 and At5g59310. At5g24770 encodes a vegetative storage protein [55], [56] and is present in two nearly identical copies. At5g59310 encodes a lipid transfer-like protein, and is a member of a small gene family in A. thaliana [57], [58].…”

The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole

“…Arabidopsis genes for vegetative storage proteins are expressed in nonseed tissues (25), and PSVs are present in protoplasts from Arabidopsis leaves (26).…”