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Abstract

Large-scale and high throughput approaches increasingly play an essential role in the study of biological systems, which are per se highly complex. Therefore, they need to be examined by these extensive methods to receive information about the large genomic and proteomic networks. In plant biology, this purpose has a strong support through the accessability of the complete genome sequence of the model plant Arabidopsis thaliana. This brief review intends to focus on the basics and the state-of-the-art of these high-throughput technologies and their application to plant proteomics. It describes protein microarrays, the use of antibodies, 2-DE and MS methods and the yeast two hybrid system, which are emerging as the major technologies for plant proteomics.

Secretome Analysis Reveals anArabidopsisLipase Involved in Defense againstAlternaria brassicicola

“…Progress in plant proteomics has largely been made possible by two-dimensional (2D) gel electrophoresisbased proteomic approaches (van Wijk, 2001;Kersten et al, 2002), and several major plant proteomics studies have focused on subcellular proteomes and protein complexes (Rouquie et al, 1997;Peltier et al, 2000;Prime et al, 2000;Kruft et al, 2001;Millar et al, 2001;Chivasa et al, 2002;Bae et al, 2003).…”

System, trends and perspectives of proteomics in dicot plantsPart I: Technologies in proteome establishment

“…This is partly due to the lack of availability of complete genomic or cDNA sequences from plants. Nevertheless, plant proteomics is gaining momentum, and is poised to become a major area of research and development in the field of plant biology (for reviews, see [10][11][12][13][14][15][16][17][18]). It has been elegantly stated by Watson and co workers "as we seek to better understand the gene function and to study the holistic biology of systems, it is inevitable that we study the proteome" [19].…”

“…Moreover, improvements in two core technologies-the classical two-dimensional gel electrophoresis (2-DGE) and mass spectrometry (MS)-have helped advance plant proteomics. There are some good reviews on plant proteomics as a whole [10][11][12][13][14][15][16][17][18], and in general deal with the proteomics of dicot plants. Therefore, this review along with two other reviews [25,26] in series is intended to cover all the studies conducted as of February 2004 on dicot plant materials from proteomics viewpoint in order to reveal the system, trends and perspectives therein.…”

Rice proteomics: Current status and future perspectives

“…The analysis of plant proteomes is now gaining a functional dimension, however, because the focus has shifted onto well-defined plant-specific tissues and organelles, the simultaneous mining of proteomic and physiological data and specific methodological efforts [17]. The first major review on plant proteomics in the years 1999 [16] and subsequently two reviews in 2001 [1,17], and recently in 2002 [2,15] have extensively covered the major technologies and contributions to plant proteomics.…”