Rice proteomics: Current status and future perspectives

Abstract

Rice proteomics: Current status and future perspectivesRice, the first cereal crop genome to be decoded, has attracted the attention of researchers worldwide because of its immense socio-economic impact on human existence. With the availability of the draft genome sequence of two major types, japonica-and indica-rice, "rice proteomics" has entered into the era of functional genomics. Although during the last decade an important but limited progress (mainly construction of protein datafiles) has been made in the field of rice proteomics, it is only recently that dedicated research groups have taken this challenge to systematically analyze the rice proteome at the cell (and organelle), tissue, and whole plant level. Important gains achieved by the accelerated technological progress in protein separation and identification will help in going beyond the simple cataloguing of rice proteins in realistic terms. In this review, we discuss the progress made in the field of rice proteomics to date and dwell upon the future direction/problems/approaches towards defining the rice proteome.

A hydroponic rice seedling culture model system for investigating proteome of salt stress in rice leaf

“…With the use of this buffer, a dramatic increase in the solubility of proteins from leaves was seen, along with reduced fragmentation of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) [33]. Moreover, as shown in the data presented here, and from previous reports [27,33,41,48], the hand-cast IEF and SDS-PAGE gels (2-DE) are reproducible and showed a clear separation of protein spots. 2-DE was performed as described in Section 2.…”

“…Since H 2 O 2 has also a toxic effect on plants, plants have evolved both enzymatic and nonenzymatic machineries to scavenge H 2 O 2 [72,[74][75][76]. As mentioned previously, immunological detection techniques could be better used when applied in conjunction with 2-DE to identify a range of proteins for which suitable antibodies are available [27]. 2-DE immunostaining was carried out using antibodies to two oxidative stress marker proteins (APX and CAT); equal amounts of protein were loaded from control and salt-treated seedling 3rd leaves (Fig.…”

“…Moreover, efficient protein solubilization with a minimum of handling is the key for a good sample preparation [27,30,33]. While using a hand-cast gel, we have used an improved LB (called LB-TT), that works well for rice (reviewed in [33]).…”

Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama

“…Guarare 8128), a necessary and proper study of the O 3 effects in these two plants was conducted. A proteomics [21][22][23][24][25][26][27][28] approach was considered for conducting an initial investigation, due to the fact that our group possesses antibodies for various ozone-responsive proteins, and second, 2-DE was previously and successfully used for investigating rice responses to O 3 [29,30]. Using both 1-DE coupled to Western analysis and 2-DE for identifying protein changes in conjunction with MS we have successfully identified protein changes in leaves of both bean and maize plants exposed to O 3 .…”

Expression and Function of Proteins during Development of the Basal Region in Rice Seedlings

“…The last decade has seen considerable progress in developing the field of rice proteomics with several studies describing the separation and characterization of proteins from various tissues or subcellular components (9,10). Several studies have dealt with the construction of proteomes for complex samples from rice, such as leaf, embryo, endosperm, root, stem, shoot, anther, and callus (11)(12)(13)(14)(15)(16)(17)(18)(19), and subcellular components such as Golgi bodies, mitochondria, plasma membrane, vacuolar membrane, Golgi membrane, and chloroplasts have also been studied (20 -22).…”