In this paper, the extraction of hyperosides from Hypericum perforatum L. using Cetyl-trimethyl-ammonium bromite (CTAB) reversed micelles was studied and the affecting factors, that is, pH value, CTAB concentration, and existence of anions in the system were comprehensively investigated. The result showed that extraction using CTAB reversed micelles is a very effective method to separate hyperoside from Hypericum perforatum L. This founding is very significant because it demonstrated that extraction using reversed micelles is a promising way to separate and purify materials of small molecules.On aétudié dans cet article l'extraction d'hyperosidesà partir d'Hypericum perforatum L.à l'aide de micelles inversées de CTAB (bromite de cétyle-triméthyle-ammonium), et on a examiné en profondeur les facteurs influents, tels la valeur de pH, la concentration de CTAB et l'existence d'anions dans le système. Les résultats montrent que l'extraction avec des micelles de CTAB inversées est une méthode très efficace pour séparer les hyperosides d'Hypericum perforatum L Cette découverte est très importante parce qu'elle démontre que l'extraction avec des micelles inversées est une façon prometteuse de séparer et de purifier des matériaux composés de petites molécules.
Keywords: extraction, hyperoside, CTAB reversed micelles
INTRODUCTIONH yperoside, also called quercetin-3-o-galactoside, is a kind of flavonoid that has many biomedical functions such as anti-allergic, anti-pastic, diuresis improving, cough relieving, blood pressure, and cholesterol reducing, protein assimilating, anti-tumor, etc. It is also widely used in medicine to relieve pain and protect cardiovascular and cerebrovascular functions (Chen et al., 1989;Song et al., 1995). Hyperosides have been found in many plants and how to separate and purify hyperosides from natural plants has attracted a lot of attention. Up to now, the only reported method to purify and separate hyperoside is alcohol extraction followed by chromatography. The general procedures are as followed: all flavonoids are first extracted using alcohol, the hyperosides are, then, separated from flavonoids using ethylacetate extraction followed by being purified by polyadipamide column chromatography. The expected hyperosides are finally obtained after concentrating, drying, and crystallization (Zhou et al., 1999;Pan et al., 2004). However, the hyperoside solutions obtained by this method contain several other flavonoids, which are very difficult to be purified. A new method is highly anticipated to produce hyperosides more effectively.Extraction using reversed micelles has been widely used in purification of biological macromolecules (Goto et al., 1999;Naoe et al., 1999; Zhang et al., 2000a;Zhou and Wong, 2006), and pharmaceutical analysis and preparation (Hu and Gulari, 1996;Su and Lee, 1999). This technology has many advantages such as high extraction efficiency, reusable surfactant and solvent, and easy to operate etc. More importantly, it does not affect the property of the target bi-molecules due...
