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Tanrıseven, Aziz; Gökmen, Fatma Özge

doi:10.1023/a:1008961016065

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…It contains different pectinolytic and cellulolytic enzymes (e.g., endo-poly-galacturonase, endo-pectinylase, and pectin esterase) (). Interestingly, Pectinex Ultra SP-L also contains a transfructosylating activity ( , ). In this work, we have assayed Pectinex Ultra SP-L, soluble or immobilized on a epoxy-activated polymethacrylate (Sepabeads EC-EP), for transformation of beet syrup and molasses into FOSs.…”

Section: Introductionmentioning

confidence: 99%

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Ghazi

Fernández‐Arrojo

Segura

et al. 2006

J. Agric. Food Chem.

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Running title: Fructo-oligosaccharides synthesis from syrup and molasses Sugar syrup and molasses from beet processing containing 620 and 570 mg/ml sucrose, 2 respectively, were assayed as low-cost and available substrates for the enzymatic synthesis 3 of fructo-oligosaccharides (FOS). A commercial pectinase (Pectinex Ultra SP-L, from 4 Aspergillus aculeatus) characterised by the presence of a transfructosylating activity, was 5 used as biocatalyst. The FOS production increased when lowering the initial pH value of 6 syrup (7.5) and molasses (8.9) to 5.5. Sugar syrup and molasses were diluted in order to 7 reduce substrate viscosity; interestingly, the percentage of FOS with regards to total sugars 8 remained almost constant, which indicated a high transferase to hydrolase ratio for this 9 enzyme. Kinetics of FOS production was analysed. Using approx. 10 U transfructosylating 10 activity per g sucrose, the FOS concentration reached a maximum of 388 mg/ml after 30 h 11 using syrup and 235 mg/ml in 65 h with molasses. These values corresponded to approx. 1256% and 49% (w/w), respectively, of the total amount of carbohydrates in the mixture. The 13 enzyme was also covalently immobilised on an epoxy-activated polymethacrylate-based 14 polymer (Sepabeads  EC-EP5). We found that immobilised Pectinex Ultra SP-L can be 15 efficiently applied to the synthesis of FOS using syrup and molasses as substrates.

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Section: Introductionmentioning

confidence: 99%

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Ghazi

Fernández‐Arrojo

Segura

et al. 2006

J. Agric. Food Chem.

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“…It contains different pectinolytic and cellulolytic enzymes (endo-poly-galacturonase, endo-pectinylase and pectin esterase) [12], and other activities such as -galactosidase [13], chitinase [14] and transgalactosidase [15]. The presence of a transfructosylating activity in Pectinex Ultra SP-L has been also reported [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides

Ghazi

Segura

Fernández‐Arrojo

et al. 2005

Journal of Molecular Catalysis B: Enzymatic

101

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The transfructosylating activity present in two commercial pectinase preparations (Pectinex Ultra SP-L, from Aspergillus aculeatus, and Rapidase TF, from Aspergillus niger) was studied. Pectinex Ultra SP-L, which has a high transferase/hydrolase ratio, was covalently immobilised on a polymethacrylate-based polymer (Sepabeads EC) activated with epoxy groups. The influence of pore volume and average pore size on biocatalyst performance was studied for two of these carriers (Sepabeads EC-EP3 and EC-EP5). Several parameters that affect immobilisation such as buffer concentration, pH and amount (mg) of protein added per gram of support (varied over the range 30:1 to 200:1) were analysed. We found that Pectinex Ultra SP-L can be efficiently immobilised on these supports without adding any external salt or buffer. Using Sepabeads EC-EP5-whose pore volume (1.67 cm 3 /g) and pore size (800 nm) are higher than those corresponding to Sepabeads EC-EP3-the activity towards sucrose reached 25.9 U/g biocatalyst. The immobilised fructosyltransferase was applied to the batch synthesis of fructo-oligosaccharides (FOS) using 630 g/l sucrose to shift activity towards transfructosylation in detriment of hydrolysis. The FOS concentration reached a maximum value of 387 g/l after 36 h (240 g/l 1-kestose, 144 g/l nystose and 3 g/l 1 Ffructofuranosyl-nystose), which corresponds to 61.5% (w/w) of the total carbohydrates in the mixture. The features of these immobilised biocatalysts are very attractive for their application in batch and fixed-bed bioreactors.

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“…A commercial pectinase preparation from Aspergillus aculeatus, Pectinase Ultra SP-L, contains FTase [51,52] besides being composed of different pectinolytic and cellulolytic enzymes. The preparation, used in the food industry to reduce the viscosity of fruit juices [42,53], was the only commercially available source of FTase according to [42].…”

Section: Food Production and Industrymentioning

confidence: 99%

Biotechnological Production of Oligosaccharides — Applications in the Food Industry

Meyer¹,

Miguel²,

Fernández³

et al. 2015

Food Production and Industry

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Oligosaccharides are carbohydrates, composed of up to twenty monosaccharides linked by glycosydic bonds, widely used in food and pharmaceutical industries. These compounds can be obtained by extraction from natural sources (milk, vegetables, fruits), and by chemical or biotechnological processes. In the last case, chemical structures and composition of the generated oligosaccharides depend on the type and source of enzymes, and on process conditions, including the initial concentration of substrate. Among the various functions of nondigestible oligosaccharides, one that has attracted attention is its prebiotic potential. The intestinal benefits of prebiotics, such as fructooligosaccharides and inulin as well as their symbiotic association with probiotic bacteria, encompass prevention and treatment of infectious diseases, including viral or bacterial diarrhea, and chronic inflammatory diseases such as ulcerative colitis. Other benefits attributed to prebiotics and probiotics include treatment of inflammatory intestinal and irritable bowel syndrome, prevention of cancer, and modulation of the immune system, mineral absorption and lipid metabolism. Fructooligosaccharides (FOS), galactooligosaccharides (GOS) and chitooligosaccharides (COS) have been widely studied for their prebiotic properties. Moreover, novel oligosaccharides with potential prebiotic activity are currently under investigation. This review will focus mainly on the biotechnological production, health benefits and applications of nonnatural oligosaccharides in the food industry.

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Cited by 13 publications

References 10 publications

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides

Biotechnological Production of Oligosaccharides — Applications in the Food Industry

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