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Reddy, M. Venkat Ram; Neelamraju, Sarla; Siddiq, E. A.

doi:10.1023/a:1020691618797

Cited by 664 publications

(78 citation statements)

References 49 publications

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“…These primers were utilised for comparative analysis of genetic diversity and for phylogenic analyses. This was in agreement with Pradeep et al (2002) who found that ISSR markers are highly polymorphic and are useful in studies on genetic diversity, phylogeny, genome mapping and evolutionary biology. Hakki et al (2007) also reported effective segregation of species with ISSR.…”

Section: Discussionsupporting

confidence: 91%

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

El-Sabrout

Aggag

2015

World Rabbit Sci.

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The importance of DNA and protein applications as powerful tools in breeding programmes is revealed. The inter simple sequence repeats (ISSR) technique was used to characterise and determine phylogenetic relationships among 4 genotypes of rabbit, namely Alexandria (Alex), V line (V), New Zealand White (NZW) and California (Cal). Six out of 7 ISSR primers exhibited sufficient variability and were used to characterise the genetic diversity and relationships among studied genotypes. A total of 141 DNA bands were detected. DNA fragments were generated with 87 (61.7%) being polymorphic, indicating considerable genetic variation among the examined genotypes. While protein electrophoresis provides a precise method for assaying variation in serum proteins which play an important part in productive performance. The results demonstrated 2 specific protein markers in Alexandria rabbits; these specific protein markers may be responsible for the superiority of Alexandria line in weight. Phylogenetic analysis based on Nei and Li unbiased genetic distance illustrated that (Alex & V) and (Cal & V) were genetically closely related. Our results showed that ISSR and protein electrophoresis are useful methods to detect different genetic expressions and understand the variability in some productive traits in rabbits.

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Section: Discussionsupporting

confidence: 91%

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

El-Sabrout

Aggag

2015

World Rabbit Sci.

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“…The Inter-Simple Sequence Repeat (ISSR) system is a very useful and efficient tool used in assessing genetic variation of plant populations. It combines the advantages of SSRs and Amplified Fragment Length Polymorphism (AFLP) to the universality of Random Polymorphic DNA (RAPD) (Reddy et al, 2002).…”

Section: Issr Analysismentioning

confidence: 99%

HEAVY METAL ANALYSIS IN RED OAK (<I>QUERCUS RUBRA</I>) POPULATIONS FROM A MINING REGION IN NORTHERN ONTARIO (CANADA): EFFECT OF SOIL LIMING AND ANALYSIS OF GENETIC VARIATION

Tran

Mehes-Smith

Narendrula

et al. 2014

American Journal of Environmental Sciences

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Understanding the dynamic of metals in soil and plants and population diversity in Northern Ontario is essential in determining progress toward ecosystem sustainability in reclaimed sites. The objectives of the present study were to assess the levels of metal content in soils and their accumulation in red oak plants from limed and unlimed sites. Genetic variation in red oak populations from the Northern Ontario region was also analyzed. The levels of soil acidity was lower in limed areas compared to un limed sites, an indication of the prolonged beneficial effect of liming 20 to 30 years ago on soil toxicity. The levels of total metals were very high for most elements, but the proportion of metals that were bio available and readily available to plants was very small. The enrichment factors were16.78, 4.98 and 2.94 for total arsenic, copper and nickel, respectively. The Translocation Factor (TF) values for available metals from soil to branches were high. There was more metal accumulation in leaves compared to branches. The degrees of genetic variability in red oak populations from limed and unlimed areas were compared using ISSR markers. The levels of polymorphic loci were moderate to high ranging from 44 to 65%. There were no significant differences in polymorphisms between areas that were limed and unlimed. Overall the red oak populations in stressed areas in Northern Ontario are sustainable.

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“…The population genetic structure can be analyzed using molecular markers, such as inter-simple sequence repeat (ISSR; Zietkiewicz et al, 1994). The polymorphism detected by this technique is binary; the technique normally detects only one allele per locus (Reddy et al, 2002). It is possible to assess the diversity of a population, as well as its spatial genetic structure using the ISSR markers.…”

Section: Introductionmentioning

confidence: 99%

Increased relatedness among the neighboring plants from seedling to adult stages in carnaúba wax palm

et al. 2016

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ABSTRACT. The objective of this study was to assess the spatial genetic structure (SGS) at different life stages (cohorts) in a remnant population (N = 101) of Copernicia prunifera in the semiarid region of northeastern Brazil. Using seven inter-simple sequence repeat molecular markers, we were able to analyze 93 loci with 100% polymorphism. Seedlings had the highest level of genetic diversity (H E = 0.411, H O = 0.599), followed by juveniles (H E = 0.394, H O = 0.579) and adults (H E = 0.267, H O = 0.427). Based on analysis of molecular variance, the majority of genetic variations were observed to occur within the life stages (93.42%) rather than between the life stages (6.58%). We found a recent reduction in the population size (bottleneck) based on the number of loci with heterozygosity excess for the two models used (infinite allele = 92 and stepwise = 91). All the life stages showed significant SGS, with positive and significant kinship values. Sp values were 0.040 for seedlings, 0.093 for juveniles, 0.156 for adults, and 0.053 for the total population. We found an increase in SGS from the seedling to adult stages, indicating that the plants were from related adult progenitors. Data from this study can be used in designing effective management and conservation strategies for the species.

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Cited by 664 publications

References 49 publications

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

HEAVY METAL ANALYSIS IN RED OAK (<I>QUERCUS RUBRA</I>) POPULATIONS FROM A MINING REGION IN NORTHERN ONTARIO (CANADA): EFFECT OF SOIL LIMING AND ANALYSIS OF GENETIC VARIATION

Increased relatedness among the neighboring plants from seedling to adult stages in carnaúba wax palm

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