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Abstract

The pharmacokinetics and residues elimination of Minocycline(MINO) were studied in healthy carp (Cyprinus carpio，400±30 g) kept at water temperatures of 10 and 25°C. The concentrations of MINO in plasma and tissues were determined using highperformance liquid chromatography (HPLC) using an ultraviolet detector. The plasma concentrations of MINO concentration-time data were fitted using a singlecompartment model at 10 and 25°C. The absorption half-life (t1/2ka) of MINO was 2.65 h at 10C and 1.65 hat 25C, whereas the elimination half-life (t1/2ke) was 11.16 h at 10 C and 16.343.78 h at 25C; the maximum plasma concentration (Cmax) and the time-point of maximum plasma concentration (Tp) were calculated as 2.34 µg mL-1 and 7.21 h at 10C, 2.97 µg mL-1 and 3.50 h at 25C. The distribution volume (Vd/F) of MINO was estimated to be 4.09 L kg-1 at 10 °C and 2.66 L kg-1 at 25 °C; the total body clearance (CLb) of MINO were computed as 0.25 and 0.49 L/ (h•kg) at 10 and 25°C, respectively; the areas under the concentration-time curve (AUC) was 59.07 µg mL-1 h at 10°C and 30.72 µg mL-1 h at 25°C. The concentrations of MINO were detected in muscle, skin, liver and kidney. The MINO could still be detected at 5 d time-point after administration at both temperatures in all tissues. The results revealed that the depletion of MINO in carp was slower with a long half-life time, especially at lower water temperature. If fish were administered MINO orally with a single dose (10 mg/kg•bw) for several days, the withdrawal periods of MINO could be not less than 19 d at winter water temperature (10℃) and 8 d at summer water temperature (25℃).

Combined Effects of Temperature and Salinity on the Pharmacokinetics of Florfenicol in Nile Tilapia (Oreochromis niloticus) Reared in Brackish Water

“…For example, increasing temperature resulted in decreased C max and increased Vd/F in Nile tilapia (20), increased C max and decreased Vd/F in crucian carp (24) and Japanese eel (23), or no change in both parameters in channel catfish (22). Other than FF, the variability in the change direction of C max and Vd/F following temperature change was seen in other antibacterial drugs as well (49)(50)(51)(52).…”