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Zavascki, Alexandre Prehn; Barth, Afonso Luís; Fernandes, Juliana Fernandez; Moro, Ana Laura Didonet; Gonçalves, Ana Lúcia Saraiva; Goldani, Luciano Zubaran

doi:10.1186/cc5006

Cited by 53 publications

(18 citation statements)

References 18 publications

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“…There are several factors related to biofilm formation, the main are: physico-chemical features of the material on which it is adhered and expression of virulence factors by the micro-organisms, as exopolymeric capsule production and fimbrial and non-fimbrial adhesins synthesis [41].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Zaranza¹,

Morais²,

Carmo³

et al. 2013

JBNB

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A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in São Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicrobial susceptibility. The most affected clinical specimens and hospital sectors were also evaluated. Most isolates were obtained from the tracheal aspirate (21.0%) and the most affected hospital sector was the ICU (43.0%). The antibiotics with the highest sensitivity rate were amikacin, piperacillin/tazobactam, fluoroquinolones, gentamicin and meropenem and the ones with the highest resistance rate were aztreonam, ceftazidime and cefepime. All samples were sensitive to polymyxin B. In relation to the expression of the gene for ESBL, 50.0% (17/34) of the multiresistant strains showed the enzyme TEM. Most strains showed high hydrophobicity and 96% of the isolates produced biofilm on a polystyrene microplate, 52% were capsule producers, 19% showed mannose-sensitive fimbriae and 39% expressed the gene algD. We observed adhesion to HEp-2 cells and to the coverslip. These factors may be reported in the pathogenesis of this bacterium, what represents a potential risk for colonization of medical devices which favor the establishment of chronic nosocomial infections.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Zaranza¹,

Morais²,

Carmo³

et al. 2013

JBNB

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“…In addition, individuals with cystic fibrosis (CF) are highly susceptible to chronic pseudomonal lung infections (5,6), and the pathogen plays a critical role in the morbidity and mortality of CF patients (7). The rate of mortality of patients infected nosocomially with P. aeruginosa is very high, with rates reported to be from ϳ30 to 50% (8)(9)(10). The emergence of clinical isolates of P. aeruginosa that exhibit resistance to one or more antibiotics, including fluoroquinolones, carbapenems, and a fourth-generation cephalosporin (cefepime) that is currently the antibiotic of choice for pseudomonal infections (11), severely limits treatment options (12)(13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa

Chen

Zhang

Davies

2017

Antimicrob Agents Chemother

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Penicillin-binding proteins (PBPs) function as transpeptidases, carboxypeptidases, or endopeptidases during peptidoglycan synthesis in bacteria. As the well-known drug targets for ␤-lactam antibiotics, the physiological functions of PBPs and whether they are essential for growth are of significant interest. The pathogen Pseudomonas aeruginosa poses a particular risk to immunocompromised and cystic fibrosis patients, and infections caused by this pathogen are difficult to treat due to antibiotic resistance. To identify potential drug targets among the PBPs in P. aeruginosa, we performed gene knockouts of all the high-molecular-mass (HMM) PBPs and determined the impacts on cell growth and morphology, susceptibility to ␤-lactams, peptidoglycan structure, virulence, and pathogenicity. Disruptions of the transpeptidase domains of most HMM PBPs, including double disruptions, had only minimal effects on cell growth. The exception was PBP3, where cell growth occurred only when the protein was conditionally expressed on an integrated plasmid. Conditional deletion of PBP3 also caused a defect in cell division and increased susceptibility to ␤-lactams. Knockout of PBP1a led to impaired motility, and this observation, together with its localization at the cell poles, suggests its involvement in flagellar function. Overall, these findings reveal that PBP3 represents the most promising target for drug discovery against P. aeruginosa, whereas other HMM PBPs have less potential.

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“…At even higher bacterial inocula, as seen in bacterial ventilator-associated pneumonia (VAP) (47), achieving high colistin concentrations against more-difficult-to-treat polymyxin-heteroresistant strains may pose a formidable challenge. Bergen et al investigated the combination of colistin plus doripenem against P. aeruginosa in vitro, simulating colistin pharmacokinetics obtained from critically ill patients (35).…”

Section: Discussionmentioning

confidence: 99%

New Dosing Strategies for an Old Antibiotic: Pharmacodynamics of Front-Loaded Regimens of Colistin at Simulated Pharmacokinetics in Patients with Kidney or Liver Disease

Rao

Haas

et al. 2014

Antimicrob Agents Chemother

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e Increasing evidence suggests that colistin monotherapy is suboptimal at currently recommended doses. We hypothesized that front-loading provides an improved dosing strategy for polymyxin antibiotics to maximize killing and minimize total exposure. Here, we utilized an in vitro pharmacodynamic model to examine the impact of front-loaded colistin regimens against a high bacterial density (10 8 CFU/ml) of Pseudomonas aeruginosa. The pharmacokinetics were simulated for patients with hepatic

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Untitled

Cited by 53 publications

References 18 publications

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa

New Dosing Strategies for an Old Antibiotic: Pharmacodynamics of Front-Loaded Regimens of Colistin at Simulated Pharmacokinetics in Patients with Kidney or Liver Disease

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Untitled

Cited by 53 publications

References 18 publications

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Strains Isolated from Clinical Samples

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Strains Isolated from Clinical Samples

Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa

New Dosing Strategies for an Old Antibiotic: Pharmacodynamics of Front-Loaded Regimens of Colistin at Simulated Pharmacokinetics in Patients with Kidney or Liver Disease

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Product

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Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples