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Burmeister, Thomas; Schwartz, Stefan; Hummel, Michael; Hoelzer, Dieter; Thiel, Eckhard

doi:10.1186/1742-4690-4-11

Cited by 8 publications

(1 citation statement)

References 34 publications

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“…An explanation for some of the negative findings ( 18 – 22 ) could be that sufficiently sensitive reagents and techniques such as PCR and sequencing were not available when the studies were conducted. Most previous studies also focused on leukocytes, the cell type involved in bovine leukemia/lymphoma ( 18 – 27 ). We focused instead on mammary epithelium, in which we had detected BLV DNA and protein in cattle ( 10 ).…”

Section: Discussionmentioning

confidence: 99%

Bovine Leukemia Virus DNA in Human Breast Tissue

Buehring¹,

Shen²,

Jensen³

et al. 2014

Emerg. Infect. Dis.

107

148

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Bovine leukemia virus (BLV), a deltaretrovirus, causes B-cell leukemia/lymphoma in cattle and is prevalent in herds globally. A previous finding of antibodies against BLV in humans led us to examine the possibility of human infection with BLV. We focused on breast tissue because, in cattle, BLV DNA and protein have been found to be more abundant in mammary epithelium than in lymphocytes. In human breast tissue specimens, we identified BLV DNA by using nested liquid-phase PCR and DNA sequencing. Variations from the bovine reference sequence were infrequent and limited to base substitutions. In situ PCR and immunohistochemical testing localized BLV to the secretory epithelium of the breast. Our finding of BLV in human tissues indicates a risk for the acquisition and proliferation of this virus in humans. Further research is needed to determine whether BLV may play a direct role in human disease.

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Section: Discussionmentioning

confidence: 99%