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Grzelak, Anna; Janiszowska, Wirginia

doi:10.1023/a:1016553909002

Cited by 17 publications

(7 citation statements)

References 20 publications

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“…Similar pattern was reported for many free triterpenoids [ 111 ]. However, there are some exceptions, for example, marigold suspension culture featured two maxima of the accumulation of oleanane-type glycosides: in the beginning of exponential and in the middle-to-the-end of stationary growth phase [ 116 ].…”

Section: Quantitative Compositionsupporting

confidence: 77%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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Section: Quantitative Compositionsupporting

confidence: 77%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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“…The coloration of the calli in this study ranged from white to yellow, orange, brown, and black, and the calli were often multicolored. Similar coloration and texture were also found in calli derived from hypocotyls [11], but the calli derived from the leaves and cotyledons were in shades of green [12]. The color of calli induced from leaves and florets also depended on sucrose concentrations in medium and was light green at lower concentrations and cream-yellow at higher concentrations [34].…”

Section: Discussionsupporting

confidence: 56%

“…In the presented work, the most effective combination of auxin and cytokinin was IAA + BAP. The combinations 2.4-D + KIN and 2.4-D + 2iP used previously [12] induced calli with much lower efficiency. Other growth hormones have proven effective for other explants such as hypocotyls, cotyledons, cotyledon nodes [11], and floret explants [34].…”

Section: Discussionmentioning

confidence: 88%

“…The induction of callus and its growth are fundamental steps for the establishment and applications of other plant tissue cultures. Callus tissues themselves can be used to prepare extracts with properties like antibiotics [11], establish cell suspensions [12], and protoplast Disclaimer/Publisher's Note: The statements, opinions, and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions, or products referred to in the content.…”

Section: Introductionmentioning

confidence: 99%

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Suspension Culture of Stem Cells Established from <em>Calendula officinalis </em>L.

Kaňuková¹,

Lenkavská²,

Gubišová³

et al. 2023

Preprint

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Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allows to consider the establishment of stem cell cultures in a broader range of species. Therefore, this study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from explants taken from leaves and roots. Appropriate combinations of plant growth regulators and light parameters for in vitro cultivation were selected. Already at this stage, stem and dedifferentiated cells could be identified, and cell suspension cultures were established from specific parts of the formed callus. Cell suspension cultures with a high proportion of stem cells originating from roots (92–93%) or leaves (72–73%) were developed. The amount of stem cells derived from the roots in the cell suspension reached a plateau at a level of 5.60–5.72105.

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“…The induction of callus and its growth are fundamental steps for the establishment and applications of other plant tissue cultures. Callus tissues themselves can be used to prepare extracts with properties like antibiotics 11 , establish cell suspensions 12 , and protoplast cultures 13 . Hairy root cultures of calendula, induced by genetic transformation with Agrobacterium rhizogenes , can also effectively produce secondary metabolites 10 , 14 – 16 .…”

Section: Introductionmentioning

confidence: 99%

Suspension culture of stem cells established of Calendula officinalis L.

Kaňuková,

Lenkavská,

Gubišová

et al. 2024

Sci Rep

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Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allow to consider the establishment of stem cell cultures in a broader range of species. This study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from leaf and root explants, and already at this stage, stem and dedifferentiated cells could be identified. The cell suspension cultures established both from the root- and leaf-derived calli contained a high proportion of stem cells (92–93% and 72–73%, respectively). The most effective combination of growth regulators for the development of stem cells in calli as well as cell cultures was 1.0 mg/L 2,4-D and 0.5 mg/L BAP. The highest amount of stem cells (5.60–5.72 × 105) was in cell suspension derived from the roots. An effective protocol for the establishment of marigold stem cell suspension culture was developed. The ratio of root-derived stem cells against dedifferentiated cells exceeded 90%.

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Untitled

Cited by 17 publications

References 20 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Suspension Culture of Stem Cells Established from <em>Calendula officinalis </em>L.

Suspension culture of stem cells established of Calendula officinalis L.

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