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Effects of homozygosity on sex determination in zebrafish Danio rerio

“…A small sample (100-200 oocytes) of the oocytes inseminated with UV-irradiated C. carpio sperm was left unshocked as a haploid control. All oocytes inseminated with UV-irradiated carp sperm (shocked and unshocked groups) were kept in the dark until the two-cell stage (45 min post-activation) in order to prevent photoreactivation of the sperm pronucleus (Ijiri & Egami, 1980;Lebeda & Flajshans, 2016Recoubratsky et al, 2003. Both D. rerio × carp hybrids and D. rerio haploids do not survive past embryonic development and so any viable larvae obtained are expected to be diploid D. rerio (Delomas & Dabrowski, 2016).…”

Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon

“…Irradiation of the entire volume of sperm inhibits spermatozoon motility and dramatically reduces fertilization capacity (Fopp-Bayat et al, 2007b;. Optimization of a sperm UV irradiation protocol is a challenge due to the high absorption rate of UV light by sperm and the wide variation in density of spermatozoa Recoubratsky et al, 2003). The reported dose of UV-C irradiation used to inactivate DNA in sturgeon sperm varies from 135 J m −2 (Recoubratsky et al, 2003) to 2838 J m −2 (Saber et al, 2008).…”

“…To increase survival rate, some protocols employ lower doses (Recoubratsky et al, 2003;Flynn et al, 2006;Fopp-Bayat, 2007) that result in only partial inactivation of DNA and the occurrence of non-gynogenetic fish among the progeny. The most common means of separating such progeny from gynogenetic is via microsatellite analysis, but this method is time consuming and laborious and requires expensive equipment, prepared primers and samples of the parent tissue.…”