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Matsumoto, K.; Vilarinhos, Alberto Duarte; Oka, Seibi

doi:10.1023/a:1016071624090

Cited by 33 publications

(3 citation statements)

References 27 publications

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“…The application of classical breeding methods has limited success in genetically improving this crop, due to the high sterility and polyploidy of most cultivated bananas. For example, the disease-resistant varieties exist particularly in non-cultivated diploids bananas (AA), but the transfer of these characters to cultivated triploid varieties (AAB or AAA) is extremely difficult by using conventional breeding methods (Matsumoto et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA)

Xiao

Huang

Gong

et al. 2009

Plant Cell Tiss Organ Cult

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To attempt to introduce genetic information of disease resistance from Musa acuminata cv. Mas (AA) to Musa silk cv. Guoshanxiang (AAB) and obtain somatic hybrids, we developed an asymmetric protoplast fusion with 20% (w/v) polyethylene glycol (PEG). The protoplasts derived from embryogenic suspension cultural cells of cv. Guoshanxiang (AAB) and cv. Mas (AA) were, respectively treated with 1.5 mM iodoacetamide (IOA) and with ultraviolet light (UV) at an intensity of 50 W/m 2 for 120 s. A total of 47 regenerated green plants were obtained and eight of which were survived in greenhouse. Six of the survived plants were identified as hybrids by RAPD analysis and only three hybrids were retained vigorously in field. The hybrid nature of the three plants was further confirmed according to their ISSR (inter-simple sequence repeat) patterns and the results indicated that they were true somatic hybrids. Chromosome analysis revealed that the three hybrids possessed an aneuploid chromosome number (2n = 34).

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Section: Introductionmentioning

confidence: 99%

“…At present, very limited successes of somatic hybridization of banana have been reported though Matsumoto et al (2002) firstly reported symmetric somatic hybridization between non-treated cv. 'Maçã' (AAB) and non-treated cv.…”

Section: Introductionmentioning

confidence: 99%

Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA)

Xiao

Huang

Gong

et al. 2009

Plant Cell Tiss Organ Cult

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“…Protoplasts have been used in studies of germplasm cryopreservation [40], somatic hybridization [41], mutant selection via somaclonal variation pathways [42,43], genetic transformant production [44] and plant-pathogen interaction [45], among others.…”

Section: Discussionmentioning

confidence: 99%

The location of aluminium in protoplasts and suspension cells taken from Coffea arabica L. with different tolerance of Al

Ramírez-Benítez

Hernández‐Sotomayor

Muñoz-Sánchez

2009

Journal of Inorganic Biochemistry

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Monocots, Tissue Culture

Neelwarne

Brettell

2010

Encyclopedia of Industrial Biotechnology

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Monocots (monocotyledonous plants) are one of the two major divisions of flowering plants, and are characterized by having parallel‐veined leaves and an embryo with a single cotyledon. Techniques of tissue and cell culture have been successfully applied to a wide range of monocot species, which include cereals and other grasses, lilies, orchids, and palms. The techniques described in this article include callus culture, cell suspension culture, regeneration of plants, protoplast culture, anther and pollen culture, and shoot tip and meristem culture. The article also covers practical applications that use technologies based on tissue and cell cultures. Examples are the rapid propagation of clonal plants, and the production of doubled haploid plants for plant breeding. Genetic transformation has also been achieved through application of tissue and cell culture techniques to grasses and cereals, bananas, pineapples, onions, garlic, and duckweeds. A further application under development is the industrial production of secondary metabolites.

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Cited by 33 publications

References 27 publications

Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA)

Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA)

The location of aluminium in protoplasts and suspension cells taken from Coffea arabica L. with different tolerance of Al

Monocots, Tissue Culture

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