99mTc-Labeled, Colistin Encapsulated, Theranostic Liposomes for Pseudomonas aeruginosa Infection

68Ga‐labeled, imatinib encapsulated, theranostic liposomes: Formulation, characterization, and in vitro evaluation of anticancer activity

“…According to our previous results (Karpuz et al, 2020(Karpuz et al, , 2023, higher encapsulation efficiency was achieved for hydrophilic drugs such as IMT using the similar rate with 8:2 (PC:Chol). Therefore, liposomes were prepared by lipid film-hydration technique by using the lipid ratios comprising 80% of phospholipid and %20 of cholesterol similar to the literature (Sun et al, 2017).…”

“…To obtain stabile 68 G8 radiolabeled liposomes, the concentration of DTPA-PE was chosen as 10% mol lipid −1 . IMT was loaded to aqueous core of liposomes due to its high hydrophilic property, and the ratio of drug to lipid was determined similarly our previous studies in which different hydrophilic drugs were used (Karpuz, Atlihan-Gundogdu, et al, 2021;Karpuz et al, 2020Karpuz et al, , 2023.…”

Design of Liposome Formulations for CRISPR/Cas9 Enzyme Immobilization: Evaluation of 5-Alpha-Reductase Enzyme Knockout for Androgenic Disorders

“…The obtained multilamellar vesicles were serially extruded 10 times through membrane filters (Whatman, USA) having 0.8, 0.6, and 0.22 μm pore sizes, respectively …”

“…The obtained multilamellar vesicles were serially extruded 10 times through membrane filters (Whatman, USA) having 0.8, 0.6, and 0.22 μm pore sizes, respectively. 31 The obtained empty formulations were evaluated in terms of their characterization properties, and stability monitoring was carried out in order to determine the optimal formulation. Cas9-loaded liposome formulation (Lipo/Cas9) was prepared by dissolving the lipid film in Cas9 enzyme solution (10 μM).…”

Nano-based theranostic approaches for infection control: current status and perspectives

“…230 When being utilized as radiolabeled nanotheranostics to deliver various antimicrobial agents, they are available for positron emission tomography imaging by introducing the radioactive isotope ions, such as 64 Cu 2+ , 99m Tc and 89 Zr. [231][232][233] Given the convenient incorporation of various agents into the structures, the lipid nanoparticles/nanoliposomes can be functionalize maltohexaose 234 or folic acid 229 for better targeting of bacteria or bacteria-infected macrophages. Notably, the construction of lauric acid and stearic acid-based thermoresponsive nanostructures with IR780 and imipenem loading and lecithin/DSPE-PEG2000 coatings could bring novel theranostic platforms with irradiation-induced phase change, leading to imipenem killing, photothermal eradication and fluorescence tracking of the MDR bacteria (Fig.…”